B6NCrl;B6N-Atm1Brd Nlktm2a(KOMP)Wtsi/CipheOrl

Status

Available to order

EMMA IDEM:09209
International strain nameB6NCrl;B6N-Atm1Brd Nlktm2a(KOMP)Wtsi/CipheOrl
Alternative nameEPD0662_3_A03
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNlktm2a(KOMP)Wtsi
Gene/Transgene symbolNlk
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Centre d'ImmunoPhenomique - Ciphe
Provider affiliationCentre d'ImmunoPhenomique - Ciphe
Genetic informationThis mouse line originates from KOMP ES clone EPD0662_3_A03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NCrl

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Hemolytic anemia due to glucophosphate isomerase deficiency / Orphanet_712
IMPC phenotypes (gene matching)
  • increased startle reflex / IMPC
  • abnormal auditory brainstem response / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (gene matching)
  • abnormal bone marrow development / MGI
  • decreased bone marrow cell number / MGI
  • spleen hypoplasia / MGI
  • decreased thymocyte number / MGI
  • ataxia / MGI
  • impaired coordination / MGI
  • impaired myelopoiesis / MGI
  • postnatal growth retardation / MGI
  • thymus hypoplasia / MGI
  • premature death / MGI
  • abnormal definitive hematopoiesis / MGI
  • no abnormal phenotype detected / MGI
  • small lymph nodes / MGI
  • abnormal immune system organ morphology / MGI
  • abnormal behavior / MGI
  • decreased lymphocyte cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased NK cell number / MGI
  • decreased pro-B cell number / MGI
  • decreased gamma-delta T cell number / MGI
  • neonatal lethality, complete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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