B6.Cg-Tg(KRT5-cre)5132Jlj/Cnbc

Status	Available to order
EMMA ID	EM:09178
International strain name	B6.Cg-Tg(KRT5-cre)5132Jlj/Cnbc
Alternative name	B6J-Tg(krt5-Cre)1Armz
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(KRT5-cre)5132Jlj
Gene/Transgene symbol	Tg(KRT5-cre)5132Jlj

Information from provider

Provider	Angel Ramirez
Provider affiliation	Molecular Oncology Unit, CIEMAT
Additional owner	José L. Jorcano. Head of the Division of Biomedicine, CIEMAT. Spain
Genetic information	Transgenic mice line with a Cre transgene regulated by regulatory elements derived from bovine keratin Krt5 gene.
Phenotypic information	Homozygous: Homozygous mice have not been generated. Heterozygous: Hemizygous mice do not show any obvious phenotype.
Breeding history	Backcrosses of transgenic mice from the original background (B6D2F2) to C57BL/6 background.
References	A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination.;Ramirez Angel, Page Angustias, Gandarillas Alberto, Zanet Jennifer, Pibre Sophie, Vidal Miguel, Tusell Laura, Genesca Anna, Whitaker Duncan A, Melton David W, Jorcano Jose L, ;2004;Genesis (New York, N.Y. : 2000);39;52-7; 15124227 Skin and hair follicle integrity is crucially dependent on beta 1 integrin expression on keratinocytes.;Brakebusch C, Grose R, Quondamatteo F, Ramirez A, Jorcano J L, Pirro A, Svensson M, Herken R, Sasaki T, Timpl R, Werner S, Fässler R, ;2000;The EMBO journal;19;3990-4003; 10921880 Conditional ablation of C/EBP beta demonstrates its keratinocyte-specific requirement for cell survival and mouse skin tumorigenesis.;Sterneck E, Zhu S, Ramirez A, Jorcano J L, Smart R C, ;2006;Oncogene;25;1272-1276; 16205634 Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues.;Berton Thomas R, Matsumoto Takashi, Page Angustias, Conti Claudio J, Deng Chu-Xia, Jorcano José L, Johnson David G, ;2003;Oncogene;22;5415-26; 12934101
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Literature references

A keratin K5Cre transgenic line appropriate for tissue-specific or generalized Cre-mediated recombination.;Ramirez Angel, Page Angustias, Gandarillas Alberto, Zanet Jennifer, Pibre Sophie, Vidal Miguel, Tusell Laura, Genesca Anna, Whitaker Duncan A, Melton David W, Jorcano Jose L, ;2004;Genesis (New York, N.Y. : 2000);39;52-7; 15124227
Skin and hair follicle integrity is crucially dependent on beta 1 integrin expression on keratinocytes.;Brakebusch C, Grose R, Quondamatteo F, Ramirez A, Jorcano J L, Pirro A, Svensson M, Herken R, Sasaki T, Timpl R, Werner S, Fässler R, ;2000;The EMBO journal;19;3990-4003; 10921880
Conditional ablation of C/EBP beta demonstrates its keratinocyte-specific requirement for cell survival and mouse skin tumorigenesis.;Sterneck E, Zhu S, Ramirez A, Jorcano J L, Smart R C, ;2006;Oncogene;25;1272-1276; 16205634
Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues.;Berton Thomas R, Matsumoto Takashi, Page Angustias, Conti Claudio J, Deng Chu-Xia, Jorcano José L, Johnson David G, ;2003;Oncogene;22;5415-26; 12934101

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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