| Status | Available to order |
| EMMA ID | EM:09169 |
| Citation information | RRID:IMSR_EM:09169 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129S4-Ddx4tm1Embrp/Cnrm |
| Alternative name | Ddx4-E457Q or Mvh E457Q |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Ddx4tm1Embrp |
| Gene/Transgene symbol | Ddx4 |
| Provider | Ramesh PILLAI |
| Provider affiliation | EMBL Grenoble Outstation, European Molecular Biology Laboratory (EMBL) |
| Genetic information | Single amino acid point mutation of the ATPase motif (DEAD to DQAD). Presence of loxP sites allows generation of conditional knock-out line. |
| Phenotypic information | Homozygous:Heterozygous knock-out mice are already shown to display male-specific infertility (Tanaka SS, et al., The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000 Apr 1;14(7):841-53). We expect our catalytic-dead point mutation of the RNA helicase domain to display similar phenotype: males are infertile in homozygous background, while females of all backgrounds and male of heterozygous background are fertile. I will let EMMA know of the phenotype as soon as I have it in a few months.Heterozygous:Heterozygous knock-out mice are already shown to display male-specific infertility (Tanaka SS, et al., The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000 Apr 1;14(7):841-53). We expect our catalytic-dead point mutation of the RNA helicase domain to display similar phenotype: males are infertile in homozygous background, while females of all backgrounds and male of heterozygous background are fertile. I will let EMMA know of the phenotype as soon as I have it in a few months. |
| Breeding history | Founder mice were crossed with wildtype C57BL/6 females. F1 generation animals are being offered for archiving to EMMA. |
| References |
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| Homozygous fertile | females only |
| Homozygous viable | females only |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
