C57BL/6N-Gatm^{tm1b(KOMP)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:09101
International strain name	C57BL/6N-Gatm^{tm1b(KOMP)Wtsi}/Ieg
Alternative name	EPD0218_5_E08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Gatm^{tm1b(KOMP)Wtsi}
Gene/Transgene symbol	Gatm
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from KOMP ES clone EPD0218_5_E08. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sor). Click here for more information on KOMP final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac, heterozygous C57BL/6NTac
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Primary Fanconi renotubular syndrome / Orphanet_3337
L-Arginine:glycine amidinotransferase deficiency / Orphanet_35704

IMPC phenotypes (allele matching)

decreased locomotor activity / IMPC
decreased exploration in new environment / IMPC
male infertility / IMPC
female infertility / IMPC
decreased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
prolonged QT interval / IMPC
increased heart rate variability / IMPC
abnormal sinus arrhythmia / IMPC
abnormal QT variability / IMPC
abnormal behavior / IMPC
decreased circulating cholesterol level / IMPC
decreased heart rate / IMPC
increased circulating aspartate transaminase level / IMPC
cardiovascular system phenotype / IMPC
increased mean corpuscular hemoglobin / IMPC
increased circulating potassium level / IMPC
increased circulating amylase level / IMPC
decreased prepulse inhibition / IMPC
decreased grip strength / IMPC
decreased red blood cell distribution width / IMPC
decreased respiratory quotient / IMPC
prolonged QRS complex duration / IMPC
prolonged RR interval / IMPC
prolonged ST segment / IMPC
decreased food intake / IMPC

IMPC phenotypes (gene matching)

prolonged RR interval / IMPC
decreased red blood cell distribution width / IMPC
male infertility / IMPC
decreased locomotor activity / IMPC
increased mean corpuscular hemoglobin / IMPC
decreased respiratory quotient / IMPC
thrombocytopenia / IMPC
decreased grip strength / IMPC
increased circulating amylase level / IMPC
decreased circulating alkaline phosphatase level / IMPC
prolonged QRS complex duration / IMPC
prolonged QT interval / IMPC
decreased prepulse inhibition / IMPC
female infertility / IMPC
decreased circulating cholesterol level / IMPC
abnormal behavior / IMPC
decreased exploration in new environment / IMPC
increased circulating aspartate transaminase level / IMPC
cardiovascular system phenotype / IMPC
decreased food intake / IMPC
increased heart rate variability / IMPC
increased circulating potassium level / IMPC
abnormal QT variability / IMPC
prolonged ST segment / IMPC
decreased heart rate / IMPC
abnormal sinus arrhythmia / IMPC

MGI phenotypes (gene matching)

scoliosis / MGI
abnormal testis morphology / MGI
abnormal spermatogenesis / MGI
decreased body length / MGI
decreased body weight / MGI
hypoactivity / MGI
abnormal eating behavior / MGI
decreased white adipose tissue amount / MGI
male infertility / MGI
female infertility / MGI
abnormal glucose homeostasis / MGI
decreased susceptibility to hepatic steatosis / MGI
decreased circulating triglyceride level / MGI
decreased circulating insulin level / MGI
abnormal gluconeogenesis / MGI
hypotonia / MGI
diarrhea / MGI
decreased circulating cholesterol level / MGI
improved glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI
growth/size/body region phenotype / MGI
immune system phenotype / MGI
decreased circulating glucose level / MGI
decreased susceptibility to diet-induced obesity / MGI
decreased circulating leptin level / MGI
decreased body mass index / MGI
increased susceptibility to induced colitis / MGI
decreased subcutaneous adipose tissue amount / MGI
increased physiological sensitivity to xenobiotic / MGI
abnormal enterocyte proliferation / MGI
increased enterocyte apoptosis / MGI
decreased liver triglyceride level / MGI
increased sensitivity to xenobiotic induced morbidity/mortality / MGI
decreased total body fat amount / MGI
decreased creatine level / MGI
increased susceptibility to weight loss / MGI
abnormal urine amino acid level / MGI
decreased urine creatinine level / MGI
abnormal duodenum crypts of Lieberkuhn morphology / MGI
decreased colon length / MGI
increased adipocyte glucose uptake / MGI
increased muscle cell glucose uptake / MGI
decreased urine creatine level / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Gatmtm1b(KOMP)Wtsi/Ieg