C57BL/6NCrl-Klk5tm2b(KOMP)Wtsi/Ph

Status

Available to order

EMMA IDEM:09056
International strain nameC57BL/6NCrl-Klk5tm2b(KOMP)Wtsi/Ph
Alternative nameEPD0151_6_H11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolKlk5tm2b(KOMP)Wtsi
Gene/Transgene symbolKlk5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Institute of Molecular Genetics
Provider affiliationDepartment of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic informationThis mouse line originates from KOMP ES clone EPD0151_6_H11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal retina morphology / IMPC
  • decreased locomotor activity / IMPC
  • decreased startle reflex / IMPC
  • increased circulating phosphate level / IMPC
  • increased mean corpuscular volume / IMPC
  • abnormal vitreous body morphology / IMPC
  • abnormal retina vasculature morphology / IMPC
  • long tibia / IMPC
  • abnormal auditory brainstem response / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • increased red blood cell distribution width / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • decreased total retina thickness / IMPC
IMPC phenotypes (gene matching)
  • abnormal auditory brainstem response / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • increased mean corpuscular volume / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • abnormal vitreous body morphology / IMPC
  • decreased total retina thickness / IMPC
  • decreased locomotor activity / IMPC
  • increased red blood cell distribution width / IMPC
  • abnormal retina vasculature morphology / IMPC
  • long tibia / IMPC
  • decreased startle reflex / IMPC
  • abnormal retina morphology / IMPC
  • increased circulating phosphate level / IMPC

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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