C57BL/6N-Lgals3tm1c(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:09044
Citation informationRRID:IMSR_EM:09044 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Lgals3tm1c(EUCOMM)Wtsi/H
Alternative nameEPD0377_1_A09
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolLgals3tm1c(EUCOMM)Wtsi
Gene/Transgene symbolLgals3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from EUCOMM ES clone EPD0377_1_A09, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)
  • increased bone mineral density / MGI
  • abnormal long bone hypertrophic chondrocyte zone / MGI
  • decreased monocyte cell number / MGI
  • decreased granulocyte number / MGI
  • abnormal liver morphology / MGI
  • abnormal hepatocyte morphology / MGI
  • increased body weight / MGI
  • aggression towards inanimate objects / MGI
  • abnormal stationary movement / MGI
  • hypoactivity / MGI
  • focal hepatic necrosis / MGI
  • impaired macrophage phagocytosis / MGI
  • brain inflammation / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • abnormal kidney physiology / MGI
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal CD8-positive, alpha-beta cytotoxic T cell morphology / MGI
  • abnormal leukocyte physiology / MGI
  • abnormal macrophage physiology / MGI
  • increased IgG level / MGI
  • abnormal social/conspecific interaction / MGI
  • abnormal monocyte morphology / MGI
  • hepatic steatosis / MGI
  • increased circulating alanine transaminase level / MGI
  • increased liver weight / MGI
  • abnormal long bone epiphyseal plate morphology / MGI
  • peritoneal inflammation / MGI
  • small intestinal inflammation / MGI
  • increased hepatocellular carcinoma incidence / MGI
  • liver fibrosis / MGI
  • decreased width of hypertrophic chondrocyte zone / MGI
  • oxidative stress / MGI
  • impaired macrophage chemotaxis / MGI
  • decreased macrophage cell number / MGI
  • increased eosinophil cell number / MGI
  • decreased lymphocyte cell number / MGI
  • abnormal response to infection / MGI
  • increased susceptibility to parasitic infection / MGI
  • sepsis / MGI
  • decreased susceptibility to injury / MGI
  • reproductive system phenotype / MGI
  • abnormal interferon secretion / MGI
  • increased tumor necrosis factor secretion / MGI
  • increased interleukin-12b secretion / MGI
  • increased interleukin-6 secretion / MGI
  • impaired neutrophil recruitment / MGI
  • increased liver triglyceride level / MGI
  • impaired cued conditioning behavior / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • increased total body fat amount / MGI
  • mortality/aging / MGI
  • integument phenotype / MGI
  • increased macrophage apoptosis / MGI
  • Mallory bodies / MGI
  • abnormal circadian behavior / MGI

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Genotyping protocol

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