C57BL/6N-Cisd2tm1c(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:09042
Citation informationRRID:IMSR_EM:09042 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Cisd2tm1c(EUCOMM)Wtsi/H
Alternative nameEPD0143_2_C03
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolCisd2tm1c(EUCOMM)Wtsi
Gene/Transgene symbolCisd2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from EUCOMM ES clone EPD0143_2_C03, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • abnormal eye size / IMPC
  • abnormal behavior / IMPC
  • decreased bone mineral density / IMPC
  • decreased circulating triglyceride level / IMPC
  • abnormal glucose homeostasis / IMPC
  • increased circulating phosphate level / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • decreased lean body mass / IMPC
  • decreased heart weight / IMPC
  • decreased circulating amylase level / IMPC
  • increased lean body mass / IMPC
  • increased total body fat amount / IMPC
  • increased bone mineral content / IMPC
  • decreased circulating creatinine level / IMPC
  • decreased locomotor activity / IMPC
  • corneal vascularization / IMPC
MGI phenotypes (gene matching)
  • prominent ears / MGI
  • decreased bone mineral density / MGI
  • kyphosis / MGI
  • lordosis / MGI
  • decreased hair follicle number / MGI
  • ruffled hair / MGI
  • tremors / MGI
  • weakness / MGI
  • muscle degeneration / MGI
  • abnormal skeletal muscle morphology / MGI
  • demyelination / MGI
  • thick epidermis / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • corneal opacity / MGI
  • abnormal optic nerve morphology / MGI
  • postnatal growth retardation / MGI
  • blindness / MGI
  • abnormal coat/hair pigmentation / MGI
  • abnormal glucose homeostasis / MGI
  • premature death / MGI
  • neurodegeneration / MGI
  • abnormal respiratory function / MGI
  • exophthalmos / MGI
  • impaired skeletal muscle contractility / MGI
  • increased insulin sensitivity / MGI
  • decreased insulin secretion / MGI
  • abnormal skeletal muscle fiber morphology / MGI
  • oliguria / MGI
  • premature aging / MGI
  • abnormal defecation / MGI
  • abnormal muscle fiber morphology / MGI
  • dilated sarcoplasmic reticulum / MGI
  • abnormal sarcomere morphology / MGI
  • abnormal calcium ion homeostasis / MGI
  • small thoracic cavity / MGI
  • lethargy / MGI
  • decreased oxygen consumption / MGI
  • impaired glucose tolerance / MGI
  • abnormal myocardium layer morphology / MGI
  • muscle phenotype / MGI
  • axon degeneration / MGI
  • decreased tidal volume / MGI
  • abnormal mitochondrion morphology / MGI
  • decreased body mass index / MGI
  • abnormal autophagy / MGI
  • decreased subcutaneous adipose tissue amount / MGI
  • retinal neovascularization / MGI
  • decreased trabecular bone thickness / MGI
  • abnormal skeletal muscle fiber type ratio / MGI
  • decreased total body fat amount / MGI
  • abnormal respiratory electron transport chain / MGI
  • increased mitochondria size / MGI
  • abnormal mitochondrial shape / MGI
  • abnormal mitochondrial crista morphology / MGI
  • decreased food intake / MGI
  • decreased fluid intake / MGI

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Practical information

Genotyping protocol

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