C57BL/6N-Bbs5tm1c(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:09033
International strain nameC57BL/6N-Bbs5tm1c(EUCOMM)Wtsi/H
Alternative nameEPD0227_3_C06
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolBbs5tm1c(EUCOMM)Wtsi
Gene/Transgene symbolBbs5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from EUCOMM ES clone EPD0227_3_C06, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • A review of standardized high-throughput cardiovascular phenotyping with a link to metabolism in mice.;Lindovsky Jiri, Nichtova Zuzana, Dragano Nathalia R V, Pajuelo Reguera David, Prochazka Jan, Fuchs Helmut, Marschall Susan, Gailus-Durner Valerie, Sedlacek Radislav, Hrabě de Angelis Martin, Rozman Jan, Spielmann Nadine, ;2023;Mammalian genome : official journal of the International Mammalian Genome Society;34;107-122; 37326672

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • increased circulating glucose level / IMPC
  • decreased bone mineral content / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased lean body mass / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal bone structure / IMPC
  • abnormal gait / IMPC
  • increased circulating fructosamine level / IMPC
  • increased total body fat amount / IMPC
  • decreased grip strength / IMPC
  • decreased bone mineral density / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • thrombocytopenia / IMPC
  • short tibia / IMPC
  • decreased respiratory quotient / IMPC
  • decreased circulating triglyceride level / IMPC
  • increased monocyte cell number / IMPC
  • increased leukocyte cell number / IMPC
  • decreased heart weight / IMPC
  • increased hematocrit / IMPC
  • increased circulating alanine transaminase level / IMPC
  • increased fasting circulating glucose level / IMPC
  • increased hemoglobin content / IMPC
  • abnormal retina morphology / IMPC
  • increased lymphocyte cell number / IMPC
  • increased mean corpuscular volume / IMPC
  • tremors / IMPC

Literature references

  • A review of standardized high-throughput cardiovascular phenotyping with a link to metabolism in mice.;Lindovsky Jiri, Nichtova Zuzana, Dragano Nathalia R V, Pajuelo Reguera David, Prochazka Jan, Fuchs Helmut, Marschall Susan, Gailus-Durner Valerie, Sedlacek Radislav, Hrabě de Angelis Martin, Rozman Jan, Spielmann Nadine, ;2023;Mammalian genome : official journal of the International Mammalian Genome Society;34;107-122; 37326672

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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