B6;FVB-Tg(tetO-GFP)2Jstr/Flmg
| Status | Available to order |
| EMMA ID | EM:09009 |
| International strain name | B6;FVB-Tg(tetO-GFP)2Jstr/Flmg |
| Alternative name | B6.FVB-Tg(tetO-GFP)2Jstr/Flmg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-GFP)2Jstr |
| Gene/Transgene symbol | Tg(tetO-GFP)2Jstr |
Information from provider
| Provider | John Strouboulis |
| Provider affiliation | Molecular Oncology, BSRC Al. Fleming |
| Additional owner | Frank Grosveld, Department of Cell Biology, Erasmus University Medical Center, Rotterdam, The Netherlands |
| Genetic information | The tetracycline operator drives GFP expression in the presence of doxycycline. Three transgenic lines carrying the Tet-inducible TRE-GFP reporter construct were generated by microinjection. Analysis of GFP expression in the liver of adult mice treated with Dox showed that TRE-GFP line 2 was induced more efficiently than TRE-GFP lines 1 and 3. Thus, TRE-GFP line 2 was retained and used as a reporter line in further studies. |
| Phenotypic information | Homozygous:no overt phenotypeHeterozygous:no overt phenotype |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous FVB/N x C57BL/6 |
Literature references
- Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island.;Katsantoni Eleni Z, Anghelescu Nora E, Rottier Robbert, Moerland Matthijs, Antoniou Michael, de Crom Rini, Grosveld Frank, Strouboulis John, ;2007;BMC developmental biology;7;108; 17900353