C57BL/6N-Msh5tm1a(EUCOMM)Wtsi/Ieg

Status

Available to order

EMMA IDEM:08955
International strain nameC57BL/6N-Msh5tm1a(EUCOMM)Wtsi/Ieg
Alternative nameEPD0638_1_B02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMsh5tm1a(EUCOMM)Wtsi
Gene/Transgene symbolMsh5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0638_1_B02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased leukocyte cell number / IMPC
  • abnormal lens morphology / IMPC
  • decreased bone mineral density / IMPC
  • increased circulating unsaturated transferrin level / IMPC
  • increased startle reflex / IMPC
MGI phenotypes (gene matching)
  • small ovary / MGI
  • small testis / MGI
  • male infertility / MGI
  • female infertility / MGI
  • ovary cysts / MGI
  • absent ovarian follicles / MGI
  • absent oocytes / MGI
  • decreased testis weight / MGI
  • azoospermia / MGI
  • abnormal female meiosis / MGI
  • abnormal male meiosis / MGI
  • decreased oocyte number / MGI
  • arrest of male meiosis / MGI
  • abnormal male germ cell apoptosis / MGI
  • increased granulosa cell tumor incidence / MGI
  • ovary degeneration / MGI
  • abnormal ovarian bursa morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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