C57BL/6NTac-Gnai2tm1(EGFP/cre/ERT2)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:08855
International strain nameC57BL/6NTac-Gnai2tm1(EGFP/cre/ERT2)Wtsi/WtsiH
Alternative nameCEPD0034_4_B06
Strain type
Allele/Transgene symbolGnai2tm1(EGFP/cre/ERT2)Wtsi
Gene/Transgene symbolGnai2

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone CEPD0034_4_B06. For further details on the construction of this clone see the page at the IMPC portal.
Quality Control/Disclaimer: The structure of the targeted mutation is not fully verified. It is recommended that the recipient confirms the mutation structure by Southern blotting. The Cre expression pattern is expected to mirror that of the host gene, but this may not be fully described and/or may be modified in the targeted allele. Although characterisation of the Cre expression pattern is an objective of EUCOMMTools this is an ongoing process which may be incomplete. It is therefore recommended that the recipient verifies the Cre expression pattern. For the tm1(EGFP/Cre/ERT2) type allele, if the promoter-driven selection cassette is flanked with Rox sites, it may be removed using Dre recombinase. Please be aware that the strain description may be subject to change. For the most recent description please consult the information pages for the appropriate product details at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • increased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • abnormal large intestine morphology / MGI
  • rectal prolapse / MGI
  • abnormal colon morphology / MGI
  • intestinal ulcer / MGI
  • abnormal immune system morphology / MGI
  • increased thymocyte number / MGI
  • decreased body weight / MGI
  • weight loss / MGI
  • abnormal digestive system physiology / MGI
  • chronic diarrhea / MGI
  • postnatal growth retardation / MGI
  • abnormal immune system physiology / MGI
  • increased inflammatory response / MGI
  • premature death / MGI
  • no abnormal phenotype detected / MGI
  • abnormal T cell physiology / MGI
  • increased immunoglobulin level / MGI
  • abnormal neutrophil physiology / MGI
  • abnormal eosinophil physiology / MGI
  • increased IgG level / MGI
  • increased IgM level / MGI
  • increased IgA level / MGI
  • increased heart rate / MGI
  • megacolon / MGI
  • colitis / MGI
  • fatigue / MGI
  • increased intestinal adenocarcinoma incidence / MGI
  • abnormal cytokine secretion / MGI
  • peritoneal inflammation / MGI
  • large intestinal inflammation / MGI
  • abnormal leukocyte adhesion / MGI
  • nervous system phenotype / MGI
  • abnormal response/metabolism to endogenous compounds / MGI
  • abnormal large intestine crypts of Lieberkuhn morphology / MGI
  • increased eosinophil cell number / MGI
  • decreased eosinophil cell number / MGI
  • increased lymphocyte cell number / MGI
  • increased T cell number / MGI
  • increased susceptibility to parasitic infection / MGI
  • decreased heart rate / MGI
  • increased T cell proliferation / MGI
  • cardiovascular system phenotype / MGI
  • immune system phenotype / MGI
  • increased single-positive T cell number / MGI
  • increased tumor necrosis factor secretion / MGI
  • increased interferon-gamma secretion / MGI
  • increased circulating interferon-gamma level / MGI
  • increased circulating interleukin-4 level / MGI
  • increased circulating interleukin-5 level / MGI
  • increased interleukin-1 beta secretion / MGI
  • increased interleukin-2 secretion / MGI
  • abnormal cytokine level / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • decreased physiological sensitivity to xenobiotic / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • abnormal cellular extravasation / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • lethality during fetal growth through weaning, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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