C57BL/6NTac-Klhl18tm1a(KOMP)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:08742
International strain nameC57BL/6NTac-Klhl18tm1a(KOMP)Wtsi/WtsiH
Alternative nameEPD0163_1_F06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolKlhl18tm1a(KOMP)Wtsi
Gene/Transgene symbolKlhl18
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0163_1_F06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Inner hair cell dysfunction in Klhl18 mutant mice leads to low frequency progressive hearing loss.;Ingham Neil J, Banafshe Navid, Panganiban Clarisse, Crunden Julia L, Chen Jing, Lewis Morag A, Steel Karen P, ;2021;PloS one;16;e0258158; 34597341

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased hematocrit / IMPC
  • decreased hemoglobin content / IMPC
  • decreased erythrocyte cell number / IMPC
  • increased grip strength / IMPC
  • increased circulating magnesium level / IMPC
IMPC phenotypes (gene matching)
  • increased grip strength / IMPC
  • decreased hematocrit / IMPC
  • increased circulating magnesium level / IMPC
  • decreased erythrocyte cell number / IMPC
  • decreased hemoglobin content / IMPC

Literature references

  • Inner hair cell dysfunction in Klhl18 mutant mice leads to low frequency progressive hearing loss.;Ingham Neil J, Banafshe Navid, Panganiban Clarisse, Crunden Julia L, Chen Jing, Lewis Morag A, Steel Karen P, ;2021;PloS one;16;e0258158; 34597341

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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