C57BL/6N-Tfap2b^{tm1a(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:08633
International strain name	C57BL/6N-Tfap2b^{tm1a(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0764_4_A09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Tfap2b^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Tfap2b
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0764_4_A09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacUSA, wild-type C57BL/6NTacUSA
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Char syndrome / Orphanet_46627
Familial patent arterial duct / Orphanet_466729

IMPC phenotypes (gene matching)

abnormal optic disk morphology / IMPC
increased grip strength / IMPC
preweaning lethality, complete penetrance / IMPC
iris synechia / IMPC

MGI phenotypes (gene matching)

decreased circulating calcium level / MGI
polydactyly / MGI
muscle spasm / MGI
tremors / MGI
scaly skin / MGI
increased circulating phosphate level / MGI
postnatal growth retardation / MGI
abnormal ion homeostasis / MGI
abnormal kidney physiology / MGI
increased circulating parathyroid hormone level / MGI
decreased urine calcium level / MGI
decreased urine osmolality / MGI
small kidney / MGI
patent ductus arteriosus / MGI
kidney cysts / MGI
increased renal tubule apoptosis / MGI
increased circulating creatinine level / MGI
increased blood urea nitrogen level / MGI
congestive heart failure / MGI
dilated proximal convoluted tubules / MGI
postaxial polydactyly / MGI
pulmonary vascular congestion / MGI
abnormal pulmonary alveolar system morphology / MGI
postnatal lethality, complete penetrance / MGI
neonatal lethality, complete penetrance / MGI
neonatal lethality, incomplete penetrance / MGI
increased kidney apoptosis / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Tfap2btm1a(EUCOMM)Wtsi/Ieg