C57BL/6N-Slc13a5^{tm1a(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:08618
International strain name	C57BL/6N-Slc13a5^{tm1a(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0667_2_B12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Slc13a5^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Slc13a5
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0667_2_B12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Pyridoxine-dependent epilepsy / Orphanet_3006
Undetermined early-onset epileptic encephalopathy / Orphanet_442835
Amelocerebrohypohidrotic syndrome / Orphanet_1946

IMPC phenotypes (gene matching)

abnormal QT variability / IMPC
increased grip strength / IMPC
short tibia / IMPC
increased leukocyte cell number / IMPC

MGI phenotypes (gene matching)

abnormal liver morphology / MGI
abnormal hepatocyte morphology / MGI
abnormal liver physiology / MGI
decreased body length / MGI
decreased body weight / MGI
hyperactivity / MGI
abnormal glucose homeostasis / MGI
abnormal muscle physiology / MGI
decreased circulating insulin level / MGI
increased insulin sensitivity / MGI
no phenotypic analysis / MGI
decreased susceptibility to age related obesity / MGI
abnormal glycerol level / MGI
increased lean body mass / MGI
increased energy expenditure / MGI
abnormal metabolism / MGI
increased oxygen consumption / MGI
improved glucose tolerance / MGI
decreased circulating glucose level / MGI
decreased susceptibility to diet-induced obesity / MGI
increased carbon dioxide production / MGI
decreased liver triglyceride level / MGI
decreased total body fat amount / MGI
abnormal hepatocyte physiology / MGI
abnormal susceptibility to weight gain / MGI
decreased circulating lactate level / MGI
increased muscle cell glucose uptake / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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