C57BL/6N-Dnase1l2^{tm1.1(KOMP)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:08574
International strain name	C57BL/6N-Dnase1l2^{tm1.1(KOMP)Wtsi}/WtsiH
Alternative name	EPD0296_2_A10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Dnase1l2^{tm1.1(KOMP)Wtsi}
Gene/Transgene symbol	Dnase1l2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0296_2_A10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
abnormal head morphology / IMPC
abnormal cranium morphology / IMPC
abnormal maxilla morphology / IMPC
abnormal stomach morphology / IMPC
abnormal tibia morphology / IMPC
abnormal autopod morphology / IMPC
abnormal nail morphology / IMPC
abnormal spleen morphology / IMPC
decreased body length / IMPC
persistence of hyaloid vascular system / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
decreased locomotor activity / IMPC
abnormal vocalization / IMPC
abnormal motor capabilities/coordination/movement / IMPC
abnormal eye morphology / IMPC
abnormal digit morphology / IMPC
abnormal tail morphology / IMPC
brachydactyly / IMPC
short tibia / IMPC
increased heart weight / IMPC
abnormal joint morphology / IMPC
abnormal bone structure / IMPC
abnormal heart left ventricle morphology / IMPC
decreased lean body mass / IMPC
abnormal spine curvature / IMPC
vertebral fusion / IMPC
fusion of vertebral arches / IMPC
increased circulating aspartate transaminase level / IMPC
increased blood urea nitrogen level / IMPC
eye hemorrhage / IMPC
abnormal optic disk morphology / IMPC
increased total body fat amount / IMPC
decreased total body fat amount / IMPC
decreased grip strength / IMPC
decreased bone mineral content / IMPC
decreased cardiac stroke volume / IMPC
increased fasting circulating glucose level / IMPC
lipid deposition / IMPC

IMPC phenotypes (gene matching)

decreased grip strength / IMPC
short tibia / IMPC
abnormal digit morphology / IMPC
decreased body length / IMPC
abnormal heart left ventricle morphology / IMPC
abnormal nail morphology / IMPC
decreased locomotor activity / IMPC
small superior vagus ganglion / IMPC
enlarged heart / IMPC
abnormal tail length / IMPC
decreased total body fat amount / IMPC
abnormal spleen morphology / IMPC
abnormal head morphology / IMPC
abnormal bone structure / IMPC
abnormal lens morphology / IMPC
fusion of vertebral arches / IMPC
abnormal tibia morphology / IMPC
abnormal joint morphology / IMPC
persistence of hyaloid vascular system / IMPC
brachydactyly / IMPC
lipid deposition / IMPC
decreased bone mineral content / IMPC
abnormal optic disk morphology / IMPC
eye hemorrhage / IMPC
vertebral fusion / IMPC
hyperactivity / IMPC
abnormal heart morphology / IMPC
abnormal cranium morphology / IMPC
cataract / IMPC
abnormal coat/hair pigmentation / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
abnormal tail morphology / IMPC
hydrometra / IMPC
decreased cardiac stroke volume / IMPC
abnormal startle reflex / IMPC
abnormal motor capabilities/coordination/movement / IMPC
abnormal coat/ hair morphology / IMPC
increased circulating calcium level / IMPC
abnormal eye morphology / IMPC
increased blood urea nitrogen level / IMPC
abnormal vocalization / IMPC
decreased lean body mass / IMPC
increased heart weight / IMPC
increased total body fat amount / IMPC
abnormal stomach morphology / IMPC
abnormal autopod morphology / IMPC
increased fasting circulating glucose level / IMPC
decreased blood urea nitrogen level / IMPC
decreased bone mineral density / IMPC
increased circulating aspartate transaminase level / IMPC
abnormal maxilla morphology / IMPC
abnormal spine curvature / IMPC

MGI phenotypes (gene matching)

abnormal nail morphology / MGI
abnormal cell nucleus morphology / MGI
fused joints / MGI
abnormal hair shaft morphology / MGI
decreased grip strength / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Dnase1l2tm1.1(KOMP)Wtsi/WtsiH