B6Brd;B6N-Tyrc-Brd Slc25a21tm1b(KOMP)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:08569
International strain nameB6Brd;B6N-Tyrc-Brd Slc25a21tm1b(KOMP)Wtsi/WtsiH
Alternative nameEPD0085_1_D04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSlc25a21tm1b(KOMP)Wtsi
Gene/Transgene symbolSlc25a21
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0085_1_D04. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using an X-linked CMV-Cre driver (EM:05414; C57BL/6N-Hprttm1(CMV-cre)Wtsi/WtsiOulu) for this purpose. Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene.;Maguire Simon, Estabel Jeanne, Ingham Neil, Pearson Selina, Ryder Edward, Carragher Damian M, Walker Nicolas, null null, Bussell James, Chan Wai-In, Keane Thomas M, Adams David J, Scudamore Cheryl L, Lelliott Christopher J, Ramírez-Solis Ramiro, Karp Natasha A, Steel Karen P, White Jacqueline K, Gerdin Anna-Karin, ;2014;PloS one;9;e91807; 24642684

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased circulating magnesium level / IMPC
  • decreased body weight / IMPC
  • abnormal mandible morphology / IMPC
  • abnormal tooth morphology / IMPC
  • abnormal cranium morphology / IMPC
  • decreased lean body mass / IMPC
  • increased total body fat amount / IMPC
  • decreased body length / IMPC
  • abnormal snout morphology / IMPC
  • abnormal joint morphology / IMPC
  • decreased bone mineral density / IMPC
  • decreased bone mineral content / IMPC
  • vertebral fusion / IMPC
  • abnormal incisor morphology / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • decreased total body fat amount / IMPC
  • absent pinna reflex / IMPC
  • decreased mean corpuscular volume / IMPC
MGI phenotypes (gene matching)
  • decreased circulating LDL cholesterol level / MGI
  • abnormal cranium morphology / MGI
  • abnormal snout morphology / MGI
  • abnormal mandible morphology / MGI
  • decreased body length / MGI
  • decreased body weight / MGI
  • abnormal tooth morphology / MGI
  • abnormal joint morphology / MGI
  • decreased circulating alanine transaminase level / MGI
  • abnormal incisor morphology / MGI
  • decreased circulating potassium level / MGI
  • decreased circulating aspartate transaminase level / MGI
  • absent pinna reflex / MGI
  • decreased total body fat amount / MGI
  • decreased bone mineral content / MGI

Literature references

  • Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene.;Maguire Simon, Estabel Jeanne, Ingham Neil, Pearson Selina, Ryder Edward, Carragher Damian M, Walker Nicolas, null null, Bussell James, Chan Wai-In, Keane Thomas M, Adams David J, Scudamore Cheryl L, Lelliott Christopher J, Ramírez-Solis Ramiro, Karp Natasha A, Steel Karen P, White Jacqueline K, Gerdin Anna-Karin, ;2014;PloS one;9;e91807; 24642684

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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