C57BL/6N-Sell^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:08544
International strain name	C57BL/6N-Sell^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0551_6_A10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sell^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Sell
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0551_6_A10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

increased spleen weight / IMPC

MGI phenotypes (gene matching)

increased monocyte cell number / MGI
decreased monocyte cell number / MGI
extramedullary hematopoiesis / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
decreased metastatic potential / MGI
decreased activated T cell number / MGI
pancreas inflammation / MGI
decreased tumor incidence / MGI
abnormal hypersensitivity reaction / MGI
abnormal dendritic cell physiology / MGI
abnormal cell-mediated immunity / MGI
abnormal neutrophil physiology / MGI
abnormal type IV hypersensitivity reaction / MGI
abnormal leukocyte migration / MGI
abnormal leukocyte adhesion / MGI
increased tumor growth/size / MGI
decreased susceptibility to autoimmune diabetes / MGI
abnormal regulatory T cell physiology / MGI
abnormal regulatory T cell number / MGI
decreased T cell proliferation / MGI
increased T cell proliferation / MGI
decreased susceptibility to autoimmune disorder / MGI
liver/biliary system phenotype / MGI
immune system phenotype / MGI
abnormal circulating protein level / MGI
decreased single-positive T cell number / MGI
lymph node hypoplasia / MGI
intermingled spleen red and white pulp / MGI
impaired neutrophil recruitment / MGI
abnormal splenic cell ratio / MGI
small popliteal lymph nodes / MGI
abnormal cellular extravasation / MGI
integument phenotype / MGI
decreased response to antigen / MGI
impaired leukocyte tethering or rolling / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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