B6NCrl;B6N-A^tm1Brd Il2ra^{tm1a(EUCOMM)Wtsi}/Orl

Status	Available to order
EMMA ID	EM:08466
International strain name	B6NCrl;B6N-A^tm1Brd Il2ra^{tm1a(EUCOMM)Wtsi}/Orl
Alternative name	EPD0822_3_A12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Il2ra^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Il2ra
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Institut de Transgenose, INTRAGENE
Provider affiliation	TAAM-CDTA UPS44, Institut de Transgenose, INTRAGENE
Genetic information	This mouse line originates from EUCOMM ES clone EPD0822_3_A12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NCrl

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Immunodeficiency due to CD25 deficiency / Orphanet_169100

IMPC phenotypes (gene matching)

prolonged PR interval / IMPC
decreased circulating cholesterol level / IMPC
decreased vertical activity / IMPC
abnormal colon morphology / IMPC
decreased erythrocyte cell number / IMPC
decreased circulating HDL cholesterol level / IMPC
abnormal duodenum morphology / IMPC
decreased circulating total protein level / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
increased circulating phosphate level / IMPC
increased large unstained cell number / IMPC
abnormal locomotor behavior / IMPC
enlarged lymph nodes / IMPC
decreased locomotor activity / IMPC
abnormal freezing behavior / IMPC
decreased circulating serum albumin level / IMPC
decreased exploration in new environment / IMPC
abnormal spleen morphology / IMPC
decreased circulating alkaline phosphatase level / IMPC
abnormal vertebrae morphology / IMPC
improved glucose tolerance / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased bone mineral density / IMPC
increased neutrophil cell number / IMPC
decreased lymphocyte cell number / IMPC
decreased circulating calcium level / IMPC
decreased circulating glucose level / IMPC
decreased mean corpuscular volume / IMPC
decreased blood urea nitrogen level / IMPC
decreased heart weight / IMPC
abnormal contextual conditioning behavior / IMPC
abnormal cholesterol homeostasis / IMPC
decreased body length / IMPC
decreased hematocrit / IMPC
increased circulating aspartate transaminase level / IMPC
decreased hemoglobin content / IMPC

MGI phenotypes (gene matching)

decreased hematocrit / MGI
abnormal salivary gland morphology / MGI
decreased salivation / MGI
lymphoid hyperplasia / MGI
enlarged spleen / MGI
enlarged lymph nodes / MGI
decreased body size / MGI
anemia / MGI
hemolytic anemia / MGI
abnormal induced morbidity/mortality / MGI
abnormal T cell activation / MGI
lung inflammation / MGI
salivary gland inflammation / MGI
premature death / MGI
abnormal lymph organ size / MGI
abnormal T cell physiology / MGI
abnormal B cell physiology / MGI
increased IgG level / MGI
increased IgA level / MGI
colitis / MGI
large intestinal inflammation / MGI
abnormal regulatory T cell physiology / MGI
increased lymphocyte cell number / MGI
diarrhea / MGI
cachexia / MGI
abnormal T cell morphology / MGI
increased memory T cell number / MGI
increased IgG1 level / MGI
increased IgG2a level / MGI
increased IgG2b level / MGI
lacrimal gland inflammation / MGI
abnormal salivary gland duct morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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