B6NCrl;B6N-A^tm1Brd Il2rb^{tm1a(EUCOMM)Hmgu}/Orl

Status	Available to order
EMMA ID	EM:08461
International strain name	B6NCrl;B6N-A^tm1Brd Il2rb^{tm1a(EUCOMM)Hmgu}/Orl
Alternative name	HEPD0592_1_F10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Il2rb^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Il2rb
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Institut de Transgenose, INTRAGENE
Provider affiliation	TAAM-CDTA UPS44, Institut de Transgenose, INTRAGENE
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0592_1_F10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NCrl

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal coat/ hair morphology / IMPC
abnormal autopod morphology / IMPC
abnormal coat appearance / IMPC
female infertility / IMPC
abnormal ear morphology / IMPC
male infertility / IMPC

MGI phenotypes (gene matching)

increased neutrophil cell number / MGI
increased granulocyte number / MGI
abnormal sex gland morphology / MGI
enlarged spleen / MGI
enlarged lymph nodes / MGI
weight loss / MGI
abnormal locomotor behavior / MGI
thymus hypoplasia / MGI
abnormal T cell activation / MGI
increased activated T cell number / MGI
liver inflammation / MGI
abnormal hair texture / MGI
premature death / MGI
abnormal spleen periarteriolar lymphoid sheath morphology / MGI
increased IgE level / MGI
abnormal lymphocyte morphology / MGI
abnormal immune system organ morphology / MGI
increased erythroid progenitor cell number / MGI
increased anti-double stranded DNA antibody level / MGI
increased anti-nuclear antigen antibody level / MGI
increased susceptibility to autoimmune hemolytic anemia / MGI
decreased regulatory T cell number / MGI
abnormal T cell clonal deletion / MGI
decreased B cell number / MGI
decreased double-positive T cell number / MGI
decreased T cell proliferation / MGI
abnormal response to transplant / MGI
abnormal common myeloid progenitor cell morphology / MGI
decreased memory T cell number / MGI
increased CD4-positive, alpha beta T cell number / MGI
increased CD8-positive, alpha-beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
increased plasma cell number / MGI
abnormal marginal zone B cell morphology / MGI
abnormal B cell activation / MGI
abnormal mononuclear cell morphology / MGI
increased IgG1 level / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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