C57BL/6N-Atm1Brd Traf2tm1a(EUCOMM)Wtsi/WtsiOrl

Status

Available to order

EMMA IDEM:08392
Citation informationRRID:IMSR_EM:08392 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Atm1Brd Traf2tm1a(EUCOMM)Wtsi/WtsiOrl
Alternative nameEPD0521_4_F03
Strain typeTargeted Mutant Strains
Allele/Transgene symbolTraf2tm1a(EUCOMM)Wtsi
Gene/Transgene symbolTraf2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0521_4_F03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NTac males

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased circulating chloride level / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • decreased alpha-beta T cell number / IMPC
IMPC phenotypes (gene matching)
  • decreased alpha-beta T cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased circulating chloride level / IMPC
MGI phenotypes (gene matching)
  • increased granulocyte number / MGI
  • decreased thymocyte number / MGI
  • decreased body size / MGI
  • lymphoid hypoplasia / MGI
  • spleen atrophy / MGI
  • thymus atrophy / MGI
  • decreased skeletal muscle mass / MGI
  • decreased lymphocyte cell number / MGI
  • decreased double-positive T cell number / MGI
  • cachexia / MGI
  • abnormal cell physiology / MGI
  • decreased immature B cell number / MGI
  • absent spleen germinal center / MGI
  • absent lymph node germinal center / MGI
  • increased circulating tumor necrosis factor level / MGI
  • increased sensitivity to induced cell death / MGI
  • increased thymocyte apoptosis / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • decreased body fat mass / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).