B6;129S6-Casp1^{tm1a(EUCOMM)Wtsi}/Oulu

Status	Available to order
EMMA ID	EM:08376
International strain name	B6;129S6-Casp1^{tm1a(EUCOMM)Wtsi}/Oulu
Alternative name	Casp1 knock-out
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Casp1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Casp1

Information from provider

Provider	Mohamed Lamkanfi
Provider affiliation	Dept. of Medical Protein Research, VIB/Ghent University, Faculty of Medicine and Health Sciences
Genetic information	The IKMC knock-out first targeting construct PG00146_Z_4_C11 was introduced into C57BL/6NCrl (male) x 129S6/SvEvTac (female) hybrid ES cells. Correct targeting was verified by PCR and Southern blotting. Chimeras were bred with C57BL/6OlaHsd mice and sperm was obtained from pups in the same background. In IVF, C57BL/6OlaHsd females were used as oocyte donors.
Phenotypic information	Homozygous: Not analyzed Heterozygous: viable
References	Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.;Van Gorp Hanne, Saavedra Pedro H V, de Vasconcelos Nathalia M, Van Opdenbosch Nina, Vande Walle Lieselotte, Matusiak Magdalena, Prencipe Giusi, Insalaco Antonella, Van Hauwermeiren Filip, Demon Dieter, Bogaert Delfien J, Dullaers Melissa, De Baere Elfride, Hochepied Tino, Dehoorne Joke, Vermaelen Karim Y, Haerynck Filomeen, De Benedetti Fabrizio, Lamkanfi Mohamed, ;2016;Proceedings of the National Academy of Sciences of the United States of America;113;14384-14389; 27911804
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd
Stage of embryos	2-cell

Literature references

Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.;Van Gorp Hanne, Saavedra Pedro H V, de Vasconcelos Nathalia M, Van Opdenbosch Nina, Vande Walle Lieselotte, Matusiak Magdalena, Prencipe Giusi, Insalaco Antonella, Van Hauwermeiren Filip, Demon Dieter, Bogaert Delfien J, Dullaers Melissa, De Baere Elfride, Hochepied Tino, Dehoorne Joke, Vermaelen Karim Y, Haerynck Filomeen, De Benedetti Fabrizio, Lamkanfi Mohamed, ;2016;Proceedings of the National Academy of Sciences of the United States of America;113;14384-14389; 27911804

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6;129S6-Casp1tm1a(EUCOMM)Wtsi/Oulu