C57BL/6N-Nlrc4^{tm1e(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:08369
International strain name	C57BL/6N-Nlrc4^{tm1e(EUCOMM)Hmgu}/H
Alternative name	HEPD0670_7_D08
Strain type	Targeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbol	Nlrc4^{tm1e(EUCOMM)Hmgu}
Gene/Transgene symbol	Nlrc4
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0670_7_D08. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Periodic fever-infantile enterocolitis-autoinflammatory syndrome / Orphanet_436166
NLRC4-related familial cold autoinflammatory syndrome / Orphanet_576349

MGI phenotypes (gene matching)

abnormal cell death / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
abnormal cytokine secretion / MGI
abnormal response to infection / MGI
immune system phenotype / MGI
decreased interleukin-1 beta secretion / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Nlrc4tm1e(EUCOMM)Hmgu/H