C57BL/6N-Prkab2tm1a(EUCOMM)Hmgu/H

Status

Available to order

EMMA IDEM:08365
International strain nameC57BL/6N-Prkab2tm1a(EUCOMM)Hmgu/H
Alternative nameHEPD0716_2_C09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPrkab2tm1a(EUCOMM)Hmgu
Gene/Transgene symbolPrkab2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0716_2_C09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased eosinophil cell number / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • decreased mean platelet volume / IMPC
MGI phenotypes (gene matching)
  • muscle weakness / MGI
  • increased circulating triglyceride level / MGI
  • abnormal glucose homeostasis / MGI
  • increased circulating insulin level / MGI
  • abnormal lipid homeostasis / MGI
  • decreased glycogen catabolism rate / MGI
  • impaired glucose tolerance / MGI
  • insulin resistance / MGI
  • decreased circulating glucose level / MGI
  • increased susceptibility to diet-induced obesity / MGI
  • decreased physiological sensitivity to xenobiotic / MGI
  • decreased skeletal muscle fiber size / MGI
  • increased total body fat amount / MGI
  • decreased liver glycogen level / MGI
  • decreased skeletal muscle glycogen level / MGI
  • impaired exercise endurance / MGI
  • increased muscle cell glucose uptake / MGI
  • decreased muscle cell glucose uptake / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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