MGI phenotypes (allele matching)
B6.129P2-Ptcratm1(icre)Hjf/Ieg
| Status | Available to order |
| EMMA ID | EM:08347 |
| International strain name | B6.129P2-Ptcratm1(icre)Hjf/Ieg |
| Alternative name | pTa-iCre knock-in |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Ptcratm1(icre)Hjf |
| Gene/Transgene symbol | Ptcra |
Information from provider
| Provider | Hans Joerg Fehling |
| Provider affiliation | University Clinics Ulm, Institute for Immunology |
| Genetic information | A mini-gene carrying a codon-optimized version of Cre recombinase (iCre) has been inserted by homologous recombination into the first exon of the Ptcra gene encoding the Pre-T-cell receptor alpha protein. The knock-in inactivates the targeted Ptcra allele. |
| Phenotypic information | Homozygous:Mice show same defects as classical Ptcra knock-out mice: partial developmental block at the transition from DN3 to DN4 stage resulting in impaired thymopoiesis of ab T cells.Heterozygous:Loss of one functional Ptcra allele; no apparent defects |
| Breeding history | The knock-in was produced in E14.1 ES cells derived from 129/Ola mice. Chimaeras were intercrossed with C57BL/6 breeding partners. Mice carrying the pTa-iCre knock-in allele were backcrossed for 20 generations onto C57BL/6. At 20th generation backcross mice were made homozygous for the knock-in allele by intercrossing heterozygous mating partners. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased thymocyte number / MGI
- thymus hypoplasia / MGI
- arrested T cell differentiation / MGI
- abnormal T cell differentiation / MGI
- abnormal double-positive T cell morphology / MGI
- no phenotypic analysis / MGI
- increased T cell number / MGI
- decreased T cell number / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased double-positive T cell number / MGI
- decreased single-positive T cell number / MGI
- increased gamma-delta T cell number / MGI
- increased DN3 thymocyte number / MGI
- decreased DN4 thymocyte number / MGI
Literature references
- In vivo fate mapping identifies pre-TCRα expression as an intra- and extrathymic, but not prethymic, marker of T lymphopoiesis.;Luche Hervé, Nageswara Rao Tata, Kumar Suresh, Tasdogan Alpaslan, Beckel Franziska, Blum Carmen, Martins Vera C, Rodewald Hans-Reimer, Fehling Hans Jörg, ;2013;The Journal of experimental medicine;210;699-714; 23509324
- The immunopathological landscape of human pre-TCRα deficiency: From rare to common variants.;Materna Marie, Delmonte Ottavia M, Bosticardo Marita, Momenilandi Mana, Conrey Peyton E, Charmeteau-De Muylder Bénédicte, Bravetti Clotilde, Bellworthy Rebecca, Cederholm Axel, Staels Frederik, Ganoza Christian A, Darko Samuel, Sayed Samir, Le Floc'h Corentin, Ogishi Masato, Rinchai Darawan, Guenoun Andrea, Bolze Alexandre, Khan Taushif, Gervais Adrian, Krüger Renate, Völler Mirjam, Palterer Boaz, Sadeghi-Shabestari Mahnaz, Langlois de Septenville Anne, Schramm Chaim A, Shah Sanjana, Tello-Cajiao John J, Pala Francesca, Amini Kayla, Campos Jose S, Lima Noemia Santana, Eriksson Daniel, Lévy Romain, Seeleuthner Yoann, Jyonouchi Soma, Ata Manar, Al Ali Fatima, Stittrich Anna, Deswarte Caroline, Pereira Anaïs, Mégret Jérôme, Le Voyer Tom, Bastard Paul, Berteloot Laureline, Dussiot Michaël, Vladikine Natasha, Cardenas Paula P, Jouanguy Emmanuelle, Alqahtani Mashael, Hasan Amal, Thanaraj Thangavel Alphonse, Rosain Jérémie, Al Qureshah Fahd, Sabato Vito, Alyanakian Marie Alexandra, Leruez-Ville Marianne, Rozenberg Flore, Haddad Elie, Regueiro Jose R, Toribio Maria L, Kelsen Judith R, Salehi Mansoor, Nasiri Shahram, Torabizadeh Mehdi, Rokni-Zadeh Hassan, Changi-Ashtiani Majid, Vatandoost Nasimeh, Moravej Hossein, Akrami Seyed Mohammad, Mazloomrezaei Mohsen, Cobat Aurélie, Meyts Isabelle, Toyofuku Etsushi, Nishimura Madoka, Moriya Kunihiko, Mizukami Tomoyuki, Imai Kohsuke, Abel Laurent, Malissen Bernard, Al-Mulla Fahd, Alkuraya Fowzan Sami, Parvaneh Nima, von Bernuth Horst, Beetz Christian, Davi Frédéric, Douek Daniel C, Cheynier Rémi, Langlais David, Landegren Nils, Marr Nico, Morio Tomohiro, Shahrooei Mohammad, Schrijvers Rik, Henrickson Sarah E, Luche Hervé, Notarangelo Luigi D, Casanova Jean-Laurent, Béziat Vivien, ;2024;Science (New York, N.Y.);383;eadh4059; 38422122