C57BL/6-Mecr^{tm1(fabI)Akast}/Oulu

Status	Available to order
EMMA ID	EM:08290
Citation information	RRID:IMSR_EM:08290 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6-Mecr^{tm1(fabI)Akast}/Oulu
Alternative name	C57BL/6-Mecrtm(fabI)/UofO
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Mecr^{tm1(fabI)Akast}
Gene/Transgene symbol	Mecr

Information from provider

Provider	Alexander Kastaniotis
Provider affiliation	Faculty of Biochemistry and Molecular Medicine, University of Oulu
Genetic information	In-frame fusion of E. coli fabI to the first 54 nucleotides of Mecr (mouse mitochondrial enoyl reductase) exon 2, generating an ORF encoding double HA-tagged bacterial fabI enoyl reductase fused to the Mecr mitochondrial targeting sequence. The fabI ORF is followed by a strong transcriptional terminator (BGH) and the neomycin resistance cassette and transcriptional terminator from loxP-FRT-PGK-gb2-neo-FRT (Gene Bridges). The neo cassette can be removed using the FRT sites.
Phenotypic information	Homozygous: Embryonic lethal at E10-11. Heterozygous: No detectable phenotype
Breeding history	ES cells and blastocysts both from C57BL/6. Chimeras were bred with C57BL/6OlaHsd females.
References	None available
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous C57BL/6JOlaHsd males, wild-type C57BL/6JOlaHsd females
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MEPAN syndrome / Orphanet_508093

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
abnormal response to tactile stimuli / IMPC
abnormal gait / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6-Mecrtm1(fabI)Akast/Oulu