IMPC phenotypes (gene matching)
C57BL/6-Mecrtm1(fabI)Akast/Oulu
| Status | Available to order |
| EMMA ID | EM:08290 |
| International strain name | C57BL/6-Mecrtm1(fabI)Akast/Oulu |
| Alternative name | C57BL/6-Mecrtm(fabI)/UofO |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Mecrtm1(fabI)Akast |
| Gene/Transgene symbol | Mecr |
Information from provider
| Provider | Alexander Kastaniotis |
| Provider affiliation | Faculty of Biochemistry and Molecular Medicine, University of Oulu |
| Genetic information | In-frame fusion of E. coli fabI to the first 54 nucleotides of Mecr (mouse mitochondrial enoyl reductase) exon 2, generating an ORF encoding double HA-tagged bacterial fabI enoyl reductase fused to the Mecr mitochondrial targeting sequence. The fabI ORF is followed by a strong transcriptional terminator (BGH) and the neomycin resistance cassette and transcriptional terminator from loxP-FRT-PGK-gb2-neo-FRT (Gene Bridges). The neo cassette can be removed using the FRT sites. |
| Phenotypic information | Homozygous:Embryonic lethal at E10-11.Heterozygous:No detectable phenotype |
| Breeding history | ES cells and blastocysts both from C57BL/6. Chimeras were bred with C57BL/6OlaHsd females. |
| References | None available |
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
| Animals used for archiving | heterozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- MEPAN syndrome / Orphanet_508093