B6.Cg-Tg(PRNP-DNAJB2_ia)61aMec/MecH
| Status | Available to order |
| EMMA ID | EM:08269 |
| International strain name | B6.Cg-Tg(PRNP-DNAJB2_ia)61aMec/MecH |
| Alternative name | DNAJB2a overexpressor transgenic line 61 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(PRNP-DNAJB2_ia)61aMec |
| Gene/Transgene symbol | Tg(PRNP-DNAJB2_ia)61aMec |
Information from provider
| Provider | Mike Cheetham |
| Provider affiliation | ORBIT, UCL Institute of Ophthalmology |
| Genetic information | This transgenic overexpressor expresses the short human isoform of DNAJB2 (DNAJB2a) also known as HSJ1a under the control of the bovine PrP promoter to drive neuronal expression. Highest levels of expression are in the nervous system with levels over 6 fold the endogenous mouse DnajB2a in the spinal cord. This line was shown to reduce G93A SOD1 aggregation and promote survival in a model of ALS - Novoselov, SS, Mustill, WJ, Gray, AL, Dick, JR, Kanuga, N, Kalmar, B, Greensmith, L and Cheetham ME (2013) Molecular chaperone mediated late-stage neuroprotection in the SOD1G93A mouse model of amyotrophic lateral sclerosis. PLoS One, 8(8):e73944, PMID: 24023695. |
| Phenotypic information | Homozygous:Mice show no obvious phenotype.Heterozygous:No obvious phenotype. This transgenic overexpressor expresses the short human isoform of DNAJB2 (DNAJB2a) also known as HSJ1a under the control of the bovine PrP promoter to drive neuronal expression. Highest levels of expression are in the nervous system with levels over 6 fold the endogenous mouse DnajB2a in the spinal cord. This line was shown to reduce G93A SOD1 aggregation and promote survival in a model of ALS - Novoselov, SS, Mustill, WJ, Gray, AL, Dick, JR, Kanuga, N, Kalmar, B, Greensmith, L and Cheetham ME (2013) Molecular chaperone mediated late-stage neuroprotection in the SOD1G93A mouse model of amyotrophic lateral sclerosis. PLoS One, 8(8):e73944, PMID: 24023695. This line has pretty constant hHSJ1a expression at a lower level than in line 52 in nearly all tissues (although spinal cord level is closer to line 52). In contrast line 52 has higher expression of hHSJ1a than this line (>2-3 fold higher) in the brain and shows a age dependent increase in expression. This line was used in the Novoselov et al. publication (2013) and line 52 was used in the Labbadia et al. publication (2012). Line 52 is also available from EMMA (EM:08268). |
| Breeding history | The animals were originally from blastocyst injections into B6xCBA cross and were back-crossed onto C57BL/6 for 4 generations before a bottleneck was suspected. A cross to B6xSJL was then made to ensure continued breeding and since then we have backcrossed over 15 generations to C57BL/6. We believe they are now congenic with C57BL/6. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Molecular chaperone mediated late-stage neuroprotection in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis.;Novoselov Sergey S, Mustill Wendy J, Gray Anna L, Dick James R, Kanuga Naheed, Kalmar Bernadett, Greensmith Linda, Cheetham Michael E, ;2013;PloS one;8;e73944; 24023695