C57BL/6N-Syktm1a(EUCOMM)Hmgu/Ieg

Status

Available to order

EMMA IDEM:08239
International strain nameC57BL/6N-Syktm1a(EUCOMM)Hmgu/Ieg
Alternative nameHEPD0722_4_A07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSyktm1a(EUCOMM)Hmgu
Gene/Transgene symbolSyk
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0722_4_A07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTacUSA, wild-type C57BL/6NTacUSA
Stage of embryos2-cell

Disease and phenotype information

IMPC phenotypes (gene matching)
  • preweaning lethality, complete penetrance / IMPC
  • decreased lean body mass / IMPC
  • decreased grip strength / IMPC
  • increased total body fat amount / IMPC
  • decreased exploration in new environment / IMPC
MGI phenotypes (gene matching)
  • increased neutrophil cell number / MGI
  • absent pre-B cells / MGI
  • decreased bone marrow cell number / MGI
  • abnormal foot pad morphology / MGI
  • abnormal lung morphology / MGI
  • abnormal blood vessel morphology / MGI
  • arrested B cell differentiation / MGI
  • decreased inflammatory response / MGI
  • abnormal lymphatic vessel morphology / MGI
  • hemorrhage / MGI
  • intracranial hemorrhage / MGI
  • intracerebral hemorrhage / MGI
  • abnormal B cell differentiation / MGI
  • hydrops fetalis / MGI
  • abnormal dendritic cell antigen presentation / MGI
  • abnormal B cell physiology / MGI
  • abnormal neutrophil physiology / MGI
  • impaired neutrophil phagocytosis / MGI
  • increased IgM level / MGI
  • glomerulonephritis / MGI
  • abnormal renal glomerular capsule morphology / MGI
  • increased anti-insulin autoantibody level / MGI
  • increased urine protein level / MGI
  • no phenotypic analysis / MGI
  • lymphatic vessel hyperplasia / MGI
  • dilated vasculature / MGI
  • abnormal brain vasculature morphology / MGI
  • decreased bone resorption / MGI
  • decreased B cell number / MGI
  • abnormal late pro-B cell / MGI
  • impaired granulocyte bactericidal activity / MGI
  • increased B cell proliferation / MGI
  • immune system phenotype / MGI
  • decreased circulating creatinine level / MGI
  • increased circulating glucose level / MGI
  • decreased blood urea nitrogen level / MGI
  • spinal hemorrhage / MGI
  • abnormal T cell number / MGI
  • abnormal NK cell differentiation / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased marginal zone B cell number / MGI
  • decreased transitional stage B cell number / MGI
  • decreased mature B cell number / MGI
  • abnormal osteoclast differentiation / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • increased tumor necrosis factor secretion / MGI
  • decreased tumor necrosis factor secretion / MGI
  • abnormal lymphangiogenesis / MGI
  • increased B cell apoptosis / MGI
  • decreased transitional stage T1 B cell number / MGI
  • decreased transitional stage T2 B cell number / MGI
  • decreased DN1 thymic pro-T cell number / MGI
  • increased DN3 thymocyte number / MGI
  • abnormal lymphatic vessel endothelium morphology / MGI
  • decreased lymphatic vessel endothelial cell number / MGI
  • enlarged lymphatic vessel / MGI
  • primary atelectasis / MGI
  • increased CD4-positive, alpha-beta memory T cell number / MGI
  • increased CD8-positive, alpha-beta memory T cell number / MGI
  • postnatal lethality, complete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • abnormal renal glomerulus basement membrane morphology / MGI
  • expanded mesangial matrix / MGI
  • mesangial cell hyperplasia / MGI
  • chylous ascites / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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