IMPC phenotypes (gene matching)
STOCK Msh5tm1Rak/Cnrm
| Status | Available to order |
| EMMA ID | EM:08236 |
| Citation information | RRID:IMSR_EM:08236 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Msh5tm1Rak/Cnrm |
| Alternative name | Msh5 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Msh5tm1Rak |
| Gene/Transgene symbol | Msh5 |
Information from provider
| Provider | Winfried Edelmann |
| Provider affiliation | Albert Einstein College of Medicine |
| Additional owner | Prof. Winfried Edelmann, Department of Cell Biology, Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, U.S.A. |
| Genetic information | The targeting construct was derived from 129 DNA BALB/c genomic DNA and it was designed to introduce a 2.0-kb BglII PGKhygro cassette within exon 18. Targeting was done in WW6 ES cells and produced a mouse with no functional Msh5 protein. |
| Phenotypic information | Msh5-/- mice are viable but sterile. Meiosis in these mice is affected due to the disruption of chromosome pairing at zygotene stage of prophase I, with apoptotic elimination at stage IV of the epithelial cell cycle. |
| Breeding history | Mice are currently inbred of the original strain provided by the author, crossed for at least 10 generations. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (gene matching)
- small ovary / MGI
- small testis / MGI
- male infertility / MGI
- female infertility / MGI
- ovary cysts / MGI
- absent ovarian follicles / MGI
- absent oocytes / MGI
- decreased testis weight / MGI
- azoospermia / MGI
- abnormal female meiosis / MGI
- abnormal male meiosis / MGI
- decreased oocyte number / MGI
- arrest of male meiosis / MGI
- abnormal male germ cell apoptosis / MGI
- increased granulosa cell tumor incidence / MGI
- ovary degeneration / MGI
- abnormal ovarian bursa morphology / MGI
Literature references
- Mammalian MutS homologue 5 is required for chromosome pairing in meiosis.;Edelmann W, Cohen P E, Kneitz B, Winand N, Lia M, Heyer J, Kolodner R, Pollard J W, Kucherlapati R, ;1999;Nature genetics;21;123-7; 9916805