C57BL/6N-A^tm1Brd Bach2^{tm1a(EUCOMM)Wtsi}/WtsiOrl

Status	Available to order
EMMA ID	EM:08168
International strain name	C57BL/6N-A^tm1Brd Bach2^{tm1a(EUCOMM)Wtsi}/WtsiOrl
Alternative name	EPD0689_1_H02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Bach2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Bach2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0689_1_H02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
increased neutrophil cell number / IMPC
increased monocyte cell number / IMPC
abnormal bone structure / IMPC
decreased regulatory T cell number / IMPC
increased eosinophil cell number / IMPC
decreased B cell number / IMPC
increased macrophage cell number / IMPC
thrombocytosis / IMPC
decreased circulating creatinine level / IMPC
increased NK cell number / IMPC
decreased follicular B cell number / IMPC
decreased mature B cell number / IMPC
increased immature B cell number / IMPC
increased circulating iron level / IMPC
decreased bone mineral content / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
increased CD8-positive, alpha-beta memory T cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased KLRG1-positive NK cell number / IMPC
increased Ly6C high monocyte number / IMPC
increased Ly6C low monocyte number / IMPC
increased KLRG1+ CD8 alpha-beta T cell number / IMPC
increased KLRG1+ CD4 alpha-beta T cell number / IMPC
increased KLRG1-positive gamma-delta T cell number / IMPC
decreased memory-marker NK cell number / IMPC
increased memory-marker NK cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased CD8-positive, naive alpha-beta T cell number / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
increased KLRG1-positive CD4-negative NK T cell number / IMPC
decreased CD11b-high dendritic cell number / IMPC
increased CD11b-low dendritic cell number / IMPC
increased CD103-positive CD11b-low dendritic cell number / IMPC
increased effector memory T-helper cell number / IMPC

IMPC phenotypes (gene matching)

increased KLRG1-positive NK cell number / IMPC
decreased mature B cell number / IMPC
abnormal optic disk morphology / IMPC
increased monocyte cell number / IMPC
increased KLRG1+ CD4 alpha-beta T cell number / IMPC
decreased transitional stage B cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased circulating creatinine level / IMPC
increased pulmonary respiratory rate / IMPC
increased KLRG1+ CD8 alpha-beta T cell number / IMPC
hyperactivity / IMPC
increased effector memory T-helper cell number / IMPC
decreased regulatory T cell number / IMPC
increased eosinophil cell number / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
decreased food intake / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased B cell number / IMPC
increased neutrophil cell number / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
decreased CD4-negative NK T cell number / IMPC
increased NK cell number / IMPC
increased memory-marker NK cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
immune system phenotype / IMPC
decreased Ly6C-positive immature NK cell number / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating glucose level / IMPC
decreased B-1a cell number / IMPC
decreased follicular B cell number / IMPC
increased CD8-positive, alpha-beta memory T cell number / IMPC
decreased exploration in new environment / IMPC
short tibia / IMPC
increased circulating amylase level / IMPC
increased CD11b-low dendritic cell number / IMPC
increased KLRG1-positive CD4-negative NK T cell number / IMPC
increased grip strength / IMPC
decreased CD11b-high dendritic cell number / IMPC
increased KLRG1-positive gamma-delta T cell number / IMPC
increased NK T cell number / IMPC
decreased bone mineral density / IMPC
decreased vertical activity / IMPC
limb grasping / IMPC
decreased prepulse inhibition / IMPC
increased CD103-positive CD11b-low dendritic cell number / IMPC
increased granulocyte number / IMPC
increased Ly6C low monocyte number / IMPC
decreased CD8-positive, naive alpha-beta T cell number / IMPC
increased circulating iron level / IMPC
increased Ly6C high monocyte number / IMPC
decreased marginal zone B cell number / IMPC
increased macrophage cell number / IMPC
decreased respiratory quotient / IMPC
decreased memory-marker NK cell number / IMPC
increased circulating aspartate transaminase level / IMPC
thrombocytosis / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
increased immature B cell number / IMPC
decreased NK T cell number / IMPC
increased circulating unsaturated transferrin level / IMPC
decreased B-2 B cell number / IMPC
decreased bone mineral content / IMPC
abnormal bone structure / IMPC

MGI phenotypes (gene matching)

spleen hypoplasia / MGI
decreased IgG level / MGI
decreased IgA level / MGI
abnormal B cell differentiation / MGI
abnormal B cell physiology / MGI
increased IgM level / MGI
abnormal somatic hypermutation frequency / MGI
abnormal class switch recombination / MGI
decreased B cell number / MGI
absent germinal center B cells / MGI
decreased marginal zone B cell number / MGI
decreased mature B cell number / MGI
increased immature B cell number / MGI
decreased IgG1 level / MGI
decreased IgG2a level / MGI
decreased IgG2b level / MGI
decreased IgG3 level / MGI
myeloid hyperplasia / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6N-Atm1Brd Bach2tm1a(EUCOMM)Wtsi/WtsiOrl