C57BL/6N-A^tm1Brd Cmip^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:08146
International strain name	C57BL/6N-A^tm1Brd Cmip^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0844_1_B08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cmip^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cmip
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0844_1_B08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Functional analysis of candidate genes from genome-wide association studies of hearing.;Ingham Neil J, Rook Victoria, Di Domenico Francesca, James Elysia, Lewis Morag A, Girotto Giorgia, Buniello Annalisa, Steel Karen P, ;2020;Hearing research;387;107879; 31927188

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

abnormal large intestine morphology / MGI
abnormal small intestine morphology / MGI
abnormal lymphatic vessel morphology / MGI
small thyroid gland / MGI
intestinal/bowel diverticulum / MGI
abnormal Mullerian duct morphology / MGI
retroesophageal right subclavian artery / MGI
abnormal liver vasculature morphology / MGI
muscular ventricular septal defect / MGI
dual inferior vena cava / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
absent segment of posterior cerebral artery / MGI
jugular vein stenosis / MGI
multiple persisting craniopharyngeal ducts / MGI
abnormal coronary sinus connection / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery topology / MGI
embryo tumor / MGI
embryo cyst / MGI

MGI phenotypes (gene matching)

abnormal large intestine morphology / MGI
abnormal small intestine morphology / MGI
abnormal lymphatic vessel morphology / MGI
no abnormal phenotype detected / MGI
small thyroid gland / MGI
intestinal/bowel diverticulum / MGI
abnormal Mullerian duct morphology / MGI
retroesophageal right subclavian artery / MGI
abnormal liver vasculature morphology / MGI
muscular ventricular septal defect / MGI
dual inferior vena cava / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
absent segment of posterior cerebral artery / MGI
jugular vein stenosis / MGI
multiple persisting craniopharyngeal ducts / MGI
abnormal coronary sinus connection / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery topology / MGI
embryo tumor / MGI
embryo cyst / MGI
persistent trigeminal artery / MGI

Literature references

Functional analysis of candidate genes from genome-wide association studies of hearing.;Ingham Neil J, Rook Victoria, Di Domenico Francesca, James Elysia, Lewis Morag A, Girotto Giorgia, Buniello Annalisa, Steel Karen P, ;2020;Hearing research;387;107879; 31927188

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Cmiptm1a(EUCOMM)Wtsi/WtsiH