C57BL/6N-A^tm1Brd Rala^{tm1a(EUCOMM)Wtsi}/WtsiCnrm

Status	Available to order
EMMA ID	EM:08144
Citation information	RRID:IMSR_EM:08144 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Rala^{tm1a(EUCOMM)Wtsi}/WtsiCnrm
Alternative name	EPD0532_3_H04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Rala^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Rala
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0532_3_H04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (allele matching)

preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC

MGI phenotypes (allele matching)

abnormal inner ear morphology / MGI
abnormal ureter morphology / MGI
abnormal forebrain morphology / MGI
abnormal olfactory bulb morphology / MGI
abnormal third ventricle morphology / MGI
abnormal hindbrain morphology / MGI
exencephaly / MGI
small superior cervical ganglion / MGI
abnormal lens morphology / MGI
intraventricular hemorrhage / MGI
abnormal brain morphology / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
retroesophageal right subclavian artery / MGI
abnormal optic stalk morphology / MGI
fusion of vertebral arches / MGI
coloboma / MGI
abnormal humerus morphology / MGI
spinal hemorrhage / MGI
abnormal cystic duct morphology / MGI
abnormal spinal cord central canal morphology / MGI
muscular ventricular septal defect / MGI
enlarged brain ventricles / MGI
abnormal vertebral artery morphology / MGI
absent segment of posterior cerebral artery / MGI
abnormal ductus venosus morphology / MGI
abnormal ductus venosus valve morphology / MGI
multiple persisting craniopharyngeal ducts / MGI
reduced sympathetic cervical ganglion size / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
anastomosis between internal carotid artery and basilar artery / MGI
abnormal vertebral artery topology / MGI
embryo cyst / MGI

MGI phenotypes (gene matching)

abnormal inner ear morphology / MGI
abnormal ureter morphology / MGI
abnormal forebrain morphology / MGI
abnormal olfactory bulb morphology / MGI
abnormal third ventricle morphology / MGI
abnormal hindbrain morphology / MGI
exencephaly / MGI
small superior cervical ganglion / MGI
abnormal lens morphology / MGI
intraventricular hemorrhage / MGI
abnormal brain morphology / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
retroesophageal right subclavian artery / MGI
abnormal optic stalk morphology / MGI
fusion of vertebral arches / MGI
coloboma / MGI
abnormal humerus morphology / MGI
cellular phenotype / MGI
spinal hemorrhage / MGI
abnormal cystic duct morphology / MGI
abnormal spinal cord central canal morphology / MGI
muscular ventricular septal defect / MGI
preweaning lethality, complete penetrance / MGI
enlarged brain ventricles / MGI
abnormal vertebral artery morphology / MGI
absent segment of posterior cerebral artery / MGI
abnormal ductus venosus morphology / MGI
abnormal ductus venosus valve morphology / MGI
multiple persisting craniopharyngeal ducts / MGI
reduced sympathetic cervical ganglion size / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
anastomosis between internal carotid artery and basilar artery / MGI
abnormal vertebral artery topology / MGI
embryo cyst / MGI
hemangioma / MGI
retrolental blood / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Ralatm1a(EUCOMM)Wtsi/WtsiCnrm