IMPC phenotypes (gene matching)
B6.C-Mcamtm1Pyu/Ieg
| Status | Available to order |
| EMMA ID | EM:08134 |
| International strain name | B6.C-Mcamtm1Pyu/Ieg |
| Alternative name | B6-Mcamtm1Pyu |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Mcamtm1Pyu |
| Gene/Transgene symbol | Mcam |
Information from provider
| Provider | Philipp Yu |
| Provider affiliation | Institute for Immunology, Philipps-Universität Marburg |
| Genetic information | Knockout of the Mcam gene. The Mcam gene deficient mice were generated by standard gene targeting techniques. Genomic sequences from Exon 11 to the middle of exon 16 were replaced by a Neomycin resistance cassette (1145bp). Gene targeting was done in a BALB/c ES cell line, after selection positive clones were tested by PCR and Southern blot and injected in C57BL/6 blastocysts. |
| Phenotypic information | None reported. Expression data available from EMMA node (provided by Prof. Judith Johnson, Institute for Immunology, Ludwig-Maximilians-Universitaet, Muenchen, Germany). |
| Breeding history | Generated in BALB/c, backcrossed 14x to C57BL/6. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6N, wild-type C57BL/6NTacUSA |
| Stage of embryos | 2-cell |