C57BL/6N-Cap2^{tm1b(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:08078
International strain name	C57BL/6N-Cap2^{tm1b(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0155_4_D08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cap2^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Cap2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0155_4_D08. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sor). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacUSA, wild-type C57BL/6NTacUSA
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Exfoliative ichthyosis / Orphanet_289586
Autosomal recessive cerebral atrophy / Orphanet_363969
Familial isolated dilated cardiomyopathy / Orphanet_154

IMPC phenotypes (allele matching)

prolonged QT interval / IMPC
decreased mean corpuscular hemoglobin / IMPC
increased red blood cell distribution width / IMPC
prolonged RR interval / IMPC
prolonged ST segment / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

increased red blood cell distribution width / IMPC
prolonged QT interval / IMPC
abnormal locomotor activation / IMPC
preweaning lethality, incomplete penetrance / IMPC
cataract / IMPC
decreased circulating triglyceride level / IMPC
prolonged ST segment / IMPC
prolonged RR interval / IMPC
decreased mean corpuscular hemoglobin / IMPC

MGI phenotypes (gene matching)

abnormal heart morphology / MGI
enlarged heart / MGI
abnormal myocardial fiber morphology / MGI
decreased body weight / MGI
microphthalmia / MGI
small pupils / MGI
postnatal growth retardation / MGI
eye inflammation / MGI
premature death / MGI
no abnormal phenotype detected / MGI
increased susceptibility to infection / MGI
dilated heart left ventricle / MGI
dilated cardiomyopathy / MGI
decreased aerobic running capacity / MGI
abnormal impulse conducting system conduction / MGI
dilated heart atrium / MGI
cardiac fibrosis / MGI
prolonged QT interval / MGI
dilated heart right atrium / MGI
abnormal sarcomere morphology / MGI
abnormal M line morphology / MGI
abnormal myocardial fiber physiology / MGI
decreased myocardial fiber number / MGI
abnormal myocardium layer morphology / MGI
decreased heart rate / MGI
homeostasis/metabolism phenotype / MGI
decreased ventricle muscle contractility / MGI
cardiac interstitial fibrosis / MGI
congestive heart failure / MGI
dilated heart ventricle / MGI
decreased survivor rate / MGI
decreased grip strength / MGI
prolonged QRS complex duration / MGI
abnormal ventricle myocardium morphology / MGI
prolonged HV interval / MGI
postnatal lethality, incomplete penetrance / MGI
prolonged PQ interval / MGI
abnormal I band morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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