C57BL/6N-Gpsm2^{tm1b(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:08055
International strain name	C57BL/6N-Gpsm2^{tm1b(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0519_5_G12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Gpsm2^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Gpsm2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0519_5_G12. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sor). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacUSA

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636
Chudley-McCullough syndrome / Orphanet_314597

IMPC phenotypes (allele matching)

abnormal lens morphology / IMPC
cataract / IMPC
abnormal retina morphology / IMPC
increased mean platelet volume / IMPC
abnormal vitreous body morphology / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal retina morphology / IMPC
abnormal vitreous body morphology / IMPC
increased mean platelet volume / IMPC
cataract / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal lens morphology / IMPC

MGI phenotypes (gene matching)

deafness / MGI
abnormal cochlear hair cell morphology / MGI
abnormal neuron specification / MGI
no phenotypic analysis / MGI
nervous system phenotype / MGI
abnormal orientation of outer hair cell stereociliary bundles / MGI
abnormal cochlear hair cell stereociliary bundle morphology / MGI
abnormal outer hair cell stereociliary bundle morphology / MGI
abnormal inner hair cell stereociliary bundle morphology / MGI
short inner hair cell stereocilia / MGI
abnormal cell differentiation / MGI
absent pinna reflex / MGI
absent startle reflex / MGI
abnormal kinocilium morphology / MGI
increased or absent threshold for auditory brainstem response / MGI
abnormal cell cytoskeleton morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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