C57BL/6N-Comt^{tm1a(EUCOMM)Wtsi}/H

Status	Available to order
EMMA ID	EM:08041
Citation information	RRID:IMSR_EM:08041 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Comt^{tm1a(EUCOMM)Wtsi}/H
Alternative name	EPD0350_1_G06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Comt^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Comt
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone EPD0350_1_G06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

increased circulating alanine transaminase level / IMPC
hyperactivity / IMPC
decreased thigmotaxis / IMPC
decreased anxiety-related response / IMPC
increased grip strength / IMPC
decreased circulating fructosamine level / IMPC
increased circulating phosphate level / IMPC
abnormal behavior / IMPC
decreased circulating total protein level / IMPC
abnormal locomotor behavior / IMPC
decreased circulating serum albumin level / IMPC

MGI phenotypes (gene matching)

hypertension / MGI
increased aggression towards males / MGI
increased anxiety-related response / MGI
increased startle reflex / MGI
decreased startle reflex / MGI
abnormal placenta morphology / MGI
abnormal homeostasis / MGI
increased dopamine level / MGI
abnormal fertility/fecundity / MGI
short gestation period / MGI
increased vertical activity / MGI
decreased vertical activity / MGI
albuminuria / MGI
abnormal fear-related response / MGI
abnormal placenta vasculature / MGI
abnormal response to novel object / MGI
abnormal spongiotrophoblast layer morphology / MGI
abnormal maternal decidual layer morphology / MGI
hypoxia / MGI
abnormal renal glomerulus morphology / MGI
behavior/neurological phenotype / MGI
abnormal spatial working memory / MGI
increased interferon-gamma secretion / MGI
abnormal xenobiotic pharmacokinetics / MGI
increased prepulse inhibition / MGI
decreased prepulse inhibition / MGI
increased uterine NK cell number / MGI
enhanced contextual conditioning behavior / MGI
impaired contextual conditioning behavior / MGI
enhanced cued conditioning behavior / MGI
impaired cued conditioning behavior / MGI
abnormal blood homeostasis / MGI
abnormal pregnancy / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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