C57BL/6NTac-Rnd3^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:08040
International strain name	C57BL/6NTac-Rnd3^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0532_5_G10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Rnd3^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Rnd3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0532_5_G10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

polycystic kidney / IMPC
decreased grip strength / IMPC
increased large unstained cell number / IMPC
enlarged heart / IMPC

MGI phenotypes (gene matching)

domed cranium / MGI
dilated third ventricle / MGI
decreased motor neuron number / MGI
convulsive seizures / MGI
abnormal neuromuscular synapse morphology / MGI
abnormal spinal nerve morphology / MGI
weight loss / MGI
delayed eyelid opening / MGI
abnormal locomotor behavior / MGI
ataxia / MGI
hypoactivity / MGI
abnormal gait / MGI
short stride length / MGI
abnormal pinna reflex / MGI
limb grasping / MGI
impaired righting response / MGI
abnormal placing response / MGI
postnatal growth retardation / MGI
hydroencephaly / MGI
abnormal reflex / MGI
abnormal motor capabilities/coordination/movement / MGI
premature death / MGI
abnormal brain ventricular system morphology / MGI
abnormal sciatic nerve morphology / MGI
cerebral aqueductal stenosis / MGI
abnormal tibialis anterior morphology / MGI
abnormal hypaxial muscle morphology / MGI
abnormal ear development / MGI
abnormal limb posture / MGI
positive geotaxis / MGI
abnormal cerebral aqueduct morphology / MGI
slow postnatal weight gain / MGI
enlarged lateral ventricles / MGI
decreased birth body size / MGI
decreased grip strength / MGI
abnormal brain ependyma morphology / MGI
postnatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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