| Status | Available to order |
| EMMA ID | EM:08029 |
| Citation information | RRID:IMSR_EM:08029 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Cdk4tm2.1Bbd/Cnbc |
| Alternative name | Cdk4 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cdk4tm2.1Bbd |
| Gene/Transgene symbol | Cdk4 |
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | An frt site was inserted 230 bp upstream of exon 2 and a PGK-hygromycin cassette flanked by frt sites was inserted 115 bp downstream of exon 4. The PGK-hygromycin cassette and exons 2-4 of Cdk4 were excised by Flp-mediated recombination. This is the resulting knock-out mouse line, after frt-mediated exon excision. |
| Phenotypic information | Homozygous:Flp-mediated excision of exons 2-4 creates a null allele for Cdk4. Homozygous mice for the null allele have smaller size, noticeable at birth and progressively more noticeable with age. Mice develop diabetes after 2 months of age due to a drastic reduction in pancreatic beta cell numbers and islet size. Complete infertility in females and partial infertility in males is also observed. Reduction in pituitary gland size and pituitary-hormones production.Heterozygous:Heterozygous mice are viable |
| Breeding history | Chimeras were crossed with CD1 females for germ line transmission analysis. Heterozygous animals for the three-frt sites allele were crossed with transgenic CAG-Flp mice to excise the frt-PGKneo-frt cassette and exons 2-4 of Cdk4. Line was maintained by inter-crossing heterozygous animals for the Cdk4 null allele. Genetic background involves 129S1/Sv, 129X1/SvJ, CD1 and C57BL/6J. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males |
