B6;CBA-Tg(Pten)1Srn/Cnbc
| Status | Available to order |
| EMMA ID | EM:08024 |
| International strain name | B6;CBA-Tg(Pten)1Srn/Cnbc |
| Alternative name | Tg(Pten)Srn or Tg.Pten[RP24-372016] or Pten |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Pten)1Srn |
| Gene/Transgene symbol | Tg(Pten)1Srn |
Information from provider
| Provider | Manuel Serrano |
| Provider affiliation | Molecular Oncology, CNIO (Spanish National Cancer Research Centre) |
| Genetic information | Transgenic strain generated by pronuclear injection of the BAC RP24-372O16 (CHORI-BACPAC Resources Center; https://bacpacresources.org/; http://www.chori.org/). Out of three founders containing the whole size BAC, one was selected for having a unique integration site. In this strain there is an increase in the gene dosage of Pten that results in a homogeneous and moderate overexpression of Pten while retaining the normal pattern of tissue expression. |
| Phenotypic information | Homozygous:unknownHeterozygous:The Pten Tg strain models the effects of a systemic pharmacological intervention aimed at enhancing Pten activity. Pten Tg mice are protected from cancer and present a significant extension of life span independent of their lower cancer incidence. They have an increased energy expenditure and protection from metabolic pathologies. The brown adipose tissue (BAT) of Pten Tg mice is hyperactive and presents high levels of the uncoupling protein Ucp1. |
| Breeding history | Transgenic founder was generated using (C57BL/6 x CBA)F1. Founder was crossed with C57BL/6J animals. The line was maintained by crossing hemizygous animals with wild-type animals of the same strain. |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous (C57BL/6 x CBA)F1 |
Literature references
- PTEN in cancer, metabolism, and aging.;Ortega-Molina Ana, Serrano Manuel, ;2013;Trends in endocrinology and metabolism: TEM;24;184-9; 23245767
- Pten positively regulates brown adipose function, energy expenditure, and longevity.;Ortega-Molina Ana, Efeyan Alejo, Lopez-Guadamillas Elena, Muñoz-Martin Maribel, Gómez-López Gonzalo, Cañamero Marta, Mulero Francisca, Pastor Joaquin, Martinez Sonia, Romanos Eduardo, Mar Gonzalez-Barroso M, Rial Eduardo, Valverde Angela M, Bischoff James R, Serrano Manuel, ;2012;Cell metabolism;15;382-94; 22405073