C57BL/6NTac-Sema4dtm1a(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:07987
Citation informationRRID:IMSR_EM:07987 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6NTac-Sema4dtm1a(EUCOMM)Wtsi/H
Alternative nameEPD0052_3_E12
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSema4dtm1a(EUCOMM)Wtsi
Gene/Transgene symbolSema4d
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0052_3_E12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • decreased bone mineral content / IMPC
  • enlarged spleen / IMPC
  • enlarged heart / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • cataract / IMPC
  • increased total body fat amount / IMPC
MGI phenotypes (gene matching)
  • abnormal immunoglobulin level / MGI
  • nervous system phenotype / MGI
  • abnormal B-1 B cell morphology / MGI
  • increased B cell number / MGI
  • decreased T cell number / MGI
  • decreased B cell proliferation / MGI
  • renal/urinary system phenotype / MGI
  • cardiovascular system phenotype / MGI
  • behavior/neurological phenotype / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased B-1a cell number / MGI
  • decreased spleen germinal center size / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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