C57BL/6NTac-Tcf7l2^{tm1a(EUCOMM)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:07858
International strain name	C57BL/6NTac-Tcf7l2^{tm1a(EUCOMM)Wtsi}/WtsiIeg
Alternative name	EPD0157_5_B10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Tcf7l2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Tcf7l2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0157_5_B10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Non-specific syndromic intellectual disability / Orphanet_528084

IMPC phenotypes (allele matching)

increased bone mineral density / IMPC
increased mean platelet volume / IMPC
increased lean body mass / IMPC
decreased total body fat amount / IMPC
increased red blood cell distribution width / IMPC
increased bone mineral content / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased lean body mass / IMPC
increased mean platelet volume / IMPC
increased red blood cell distribution width / IMPC
preweaning lethality, complete penetrance / IMPC
increased bone mineral density / IMPC
increased bone mineral content / IMPC
decreased total body fat amount / IMPC

MGI phenotypes (allele matching)

abnormal glucose homeostasis / MGI
abnormal response/metabolism to endogenous compounds / MGI
impaired glucose tolerance / MGI
increased circulating glucose level / MGI
abnormal hepatocyte physiology / MGI
lethality, complete penetrance / MGI
double outlet right ventricle / MGI
absent kidney / MGI
enlarged liver sinusoidal spaces / MGI
abnormal forebrain morphology / MGI
abnormal nasal septum morphology / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
retroesophageal right subclavian artery / MGI
abnormal neurohypophysis morphology / MGI
basisphenoid bone foramen / MGI
vertebral fusion / MGI
fusion of vertebral arches / MGI
abnormal inferior vena cava morphology / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
common truncal valve / MGI
abnormal pulmonary valve cusp morphology / MGI
abnormal umbilical vein morphology / MGI
abnormal vertebral artery morphology / MGI
abnormal basilar artery morphology / MGI
abnormal cecum position / MGI
absent ductus venosus valve / MGI
abnormal ureter topology / MGI
abnormal elbow joint morphology / MGI
symmetric azygos veins / MGI
abnormal umbilical vein topology / MGI
abnormal vertebral artery topology / MGI
heterochrony / MGI

MGI phenotypes (gene matching)

hypoglycemia / MGI
double outlet right ventricle / MGI
decreased enterocyte cell number / MGI
abnormal intestine morphology / MGI
abnormal enterocyte morphology / MGI
abnormal intestinal epithelium morphology / MGI
abnormal crypts of Lieberkuhn morphology / MGI
abnormal colon morphology / MGI
abnormal small intestine morphology / MGI
absent kidney / MGI
enlarged liver sinusoidal spaces / MGI
enlarged pituitary gland / MGI
abnormal forebrain morphology / MGI
decreased body weight / MGI
hypoactivity / MGI
polyphagia / MGI
abnormal glucose homeostasis / MGI
abnormal nasal septum morphology / MGI
decreased circulating insulin level / MGI
abnormal intestinal goblet cell morphology / MGI
abnormal response/metabolism to endogenous compounds / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
abnormal craniofacial development / MGI
decreased lean body mass / MGI
retroesophageal right subclavian artery / MGI
abnormal neurohypophysis morphology / MGI
basisphenoid bone foramen / MGI
vertebral fusion / MGI
fusion of vertebral arches / MGI
improved glucose tolerance / MGI
impaired glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI
cellular phenotype / MGI
increased circulating glucose level / MGI
decreased circulating glucose level / MGI
abnormal inferior vena cava morphology / MGI
abnormal pituitary intermediate lobe morphology / MGI
enlarged adenohypophysis / MGI
abnormal enterocyte proliferation / MGI
absent gastric milk in neonates / MGI
decreased birth weight / MGI
decreased birth body size / MGI
decreased total body fat amount / MGI
abnormal hepatocyte physiology / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
common truncal valve / MGI
abnormal pulmonary valve cusp morphology / MGI
abnormal intestinal enteroendocrine cell morphology / MGI
neonatal lethality, complete penetrance / MGI
lethality, complete penetrance / MGI
abnormal vertebral artery morphology / MGI
abnormal basilar artery morphology / MGI
embryo tissue necrosis / MGI
abnormal small intestine goblet cell morphology / MGI
abnormal colon goblet cell morphology / MGI
abnormal cecum position / MGI
absent ductus venosus valve / MGI
duplication of ductus venosus / MGI
abnormal ureter topology / MGI
abnormal elbow joint morphology / MGI
symmetric azygos veins / MGI
abnormal umbilical vein topology / MGI
abnormal vertebral artery topology / MGI
heterochrony / MGI
abnormal inferior vena cava topology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Tcf7l2tm1a(EUCOMM)Wtsi/WtsiIeg