C57BL/6N-Atm1Brd Card9tm1a(EUCOMM)Hmgu/WtsiOrl

Status

Available to order

EMMA IDEM:07856
International strain nameC57BL/6N-Atm1Brd Card9tm1a(EUCOMM)Hmgu/WtsiOrl
Alternative nameHEPD0678_2_G09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolCard9tm1a(EUCOMM)Hmgu
Gene/Transgene symbolCard9
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0678_2_G09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6N Tac
Breeding at archiving centreThe old batch of frozen sperm has been done with the Jackson Lab. protocol. The new batch has been done with the Ferti-up + CARD protocol.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Predisposition to invasive fungal disease due to CARD9 deficiency / Orphanet_457088
MGI phenotypes (gene matching)
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal innate immunity / MGI
  • abnormal macrophage physiology / MGI
  • abnormal cytokine secretion / MGI
  • immune system phenotype / MGI
  • increased susceptibility to fungal infection / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased interleukin-10 secretion / MGI
  • decreased interleukin-12b secretion / MGI
  • decreased interleukin-23 secretion / MGI
  • decreased interleukin-6 secretion / MGI
  • abnormal cytokine level / MGI
  • abnormal chemokine secretion / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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