B6(Cg)-Map3k14aly/Ieg

Status

Available to order

EMMA IDEM:07800
Citation informationRRID:IMSR_EM:07800 

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International strain nameB6(Cg)-Map3k14aly/Ieg
Alternative nameC57BL/6J-Map3k14 N12
Strain typeSpontaneous
Allele/Transgene symbolMap3k14aly
Gene/Transgene symbolMap3k14

Information from provider

ProviderJoerg Kirberg
Provider affiliationDiv. of Immunology (3/3), Paul-Ehrlich-Institut
Genetic informationMice carry the known mutation of Map3k14 (mitogen-activated protein kinase kinase kinase 14, aka NIK, NF-kB inducing kinase) termed aly (alymphoplasia). That mutation arose spontaneously on a mixed background (AEJ/GnRk * C57BL/6J; The Jackson Lab ref. J:16865, PubMed ref. PMID 8299692) and was later identified as G-to-A transition point mutation resulting in a replacement of a conserved glycine for an arginine at position 855 of the encoded protein (The Jackson Lab ref. J:54529; PubMed ref. PMID 10319865). The mutant allele was reported to be bred to C57BL/6. However, variations in the proportions of marginal B cells in the spleen, reported to be absent in aly mice, were observed here. For this reason, the mutant allele was backcrossed to C57BL/6 for 12 generations (exchanging the sex chromosomes along).
Phenotypic informationHomozygous:
Alymphoplasia, see The Jackson Lab ref. J:16865, PubMed ref. PMID 8299692. Underlying mutation: see The Jackson Lab ref. J:54529, PubMed ref. PMID 10319865.

Heterozygous:
nothing obvious
Breeding historyThe mutant allele was backcrossed to C57BL/6 for 12 generations (exchanging the sex chromosomes along). Following this, the line was expanded by intercrossing to obtain aly/aly homozygotes. Since aly/aly females are supposedly unable to lactate, the line is maintained as aly heterozygous females mated with aly/aly homozygous males.
References
  • A new mutation, aly, that induces a generalized lack of lymph nodes accompanied by immunodeficiency in mice.;Miyawaki S, Nakamura Y, Suzuka H, Koba M, Yasumizu R, Ikehara S, Shibata Y, ;1994;European journal of immunology;24;429-34; 8299692
  • Alymphoplasia is caused by a point mutation in the mouse gene encoding Nf-kappa b-inducing kinase.;Shinkura R, Kitada K, Matsuda F, Tashiro K, Ikuta K, Suzuki M, Kogishi K, Serikawa T, Honjo T, ;1999;Nature genetics;22;74-7; 10319865
Homozygous fertilemales only
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedyes

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6J males, wild-type C57BL/6J females
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • premature death / MGI
  • increased susceptibility to infection / MGI
  • absent lymph nodes / MGI
  • decreased mast cell number / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal salivary gland morphology / MGI
  • abnormal mammary gland development / MGI
  • abnormal immune system morphology / MGI
  • small thymus / MGI
  • abnormal immune system physiology / MGI
  • abnormal T cell activation / MGI
  • increased inflammatory response / MGI
  • kidney inflammation / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • pancreas inflammation / MGI
  • abnormal hepatobiliary system morphology / MGI
  • abnormal thymus medulla morphology / MGI
  • abnormal dendritic cell physiology / MGI
  • abnormal dendritic cell antigen presentation / MGI
  • decreased IgE level / MGI
  • absent Peyer's patches / MGI
  • abnormal bile duct morphology / MGI
  • dilated bile duct / MGI
  • bile duct inflammation / MGI
  • bile duct proliferation / MGI
  • abnormal intestinal goblet cell morphology / MGI
  • bile duct hyperplasia / MGI
  • decreased regulatory T cell number / MGI
  • increased eosinophil cell number / MGI
  • increased susceptibility to parasitic infection / MGI
  • abnormal NK T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased dendritic cell number / MGI
  • abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology / MGI
  • decreased circulating interferon-gamma level / MGI
  • decreased circulating interleukin-4 level / MGI
  • lacrimal gland inflammation / MGI
  • abnormal mammary fat pad morphology / MGI
  • decreased mammary fat pad weight / MGI
  • abnormal thymus corticomedullary boundary morphology / MGI
  • thymus cysts / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal lymphocyte morphology / MGI
  • increased length of allograft survival / MGI
  • decreased B cell number / MGI
  • absent follicular dendritic cells / MGI
  • absent spleen marginal zone / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • abnormal spleen primary B follicle morphology / MGI
  • mortality/aging / MGI
  • absent thymus corticomedullary boundary / MGI
MGI phenotypes (gene matching)
  • decreased mast cell number / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal salivary gland morphology / MGI
  • abnormal mammary gland development / MGI
  • abnormal immune system morphology / MGI
  • abnormal spleen morphology / MGI
  • abnormal thymus morphology / MGI
  • small thymus / MGI
  • abnormal immune system physiology / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • abnormal T cell activation / MGI
  • increased inflammatory response / MGI
  • kidney inflammation / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • pancreas inflammation / MGI
  • premature death / MGI
  • abnormal hepatobiliary system morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • abnormal thymus medulla morphology / MGI
  • abnormal dendritic cell physiology / MGI
  • abnormal lymphopoiesis / MGI
  • increased susceptibility to infection / MGI
  • abnormal dendritic cell antigen presentation / MGI
  • abnormal B cell physiology / MGI
  • decreased immunoglobulin level / MGI
  • decreased IgE level / MGI
  • abnormal lymphocyte morphology / MGI
  • absent Peyer's patches / MGI
  • abnormal bile duct morphology / MGI
  • dilated bile duct / MGI
  • bile duct inflammation / MGI
  • bile duct proliferation / MGI
  • abnormal intestinal goblet cell morphology / MGI
  • increased length of allograft survival / MGI
  • abnormal class switch recombination / MGI
  • bile duct hyperplasia / MGI
  • decreased regulatory T cell number / MGI
  • increased eosinophil cell number / MGI
  • decreased B cell number / MGI
  • increased susceptibility to parasitic infection / MGI
  • absent lymph nodes / MGI
  • abnormal NK T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased dendritic cell number / MGI
  • decreased follicular B cell number / MGI
  • decreased germinal center B cell number / MGI
  • decreased marginal zone B cell number / MGI
  • absent follicular dendritic cells / MGI
  • decreased B-2 B cell number / MGI
  • abnormal B cell activation / MGI
  • absent spleen marginal zone / MGI
  • abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology / MGI
  • absent mesenteric lymph nodes / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • abnormal spleen primary B follicle morphology / MGI
  • decreased IgG2b level / MGI
  • decreased circulating interferon-gamma level / MGI
  • decreased circulating interleukin-4 level / MGI
  • abnormal thymus cell ratio / MGI
  • lacrimal gland inflammation / MGI
  • abnormal mammary fat pad morphology / MGI
  • decreased mammary fat pad weight / MGI
  • abnormal thymus corticomedullary boundary morphology / MGI
  • absent inguinal lymph nodes / MGI
  • small brachial lymph nodes / MGI
  • absent axillary lymph nodes / MGI
  • small axillary lymph nodes / MGI
  • absent cervical lymph nodes / MGI
  • absent popliteal lymph nodes / MGI
  • decreased transitional stage T1 B cell number / MGI
  • decreased transitional stage T2 B cell number / MGI
  • mortality/aging / MGI
  • absent thymus corticomedullary boundary / MGI
  • thymus cysts / MGI

Literature references

  • A new mutation, aly, that induces a generalized lack of lymph nodes accompanied by immunodeficiency in mice.;Miyawaki S, Nakamura Y, Suzuka H, Koba M, Yasumizu R, Ikehara S, Shibata Y, ;1994;European journal of immunology;24;429-34; 8299692
  • Alymphoplasia is caused by a point mutation in the mouse gene encoding Nf-kappa b-inducing kinase.;Shinkura R, Kitada K, Matsuda F, Tashiro K, Ikuta K, Suzuki M, Kogishi K, Serikawa T, Honjo T, ;1999;Nature genetics;22;74-7; 10319865

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