C57BL/6N-Ppt2^{tm2a(KOMP)Wtsi}/H

Status	Available to order
EMMA ID	EM:07784
Citation information	RRID:IMSR_EM:07784 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Ppt2^{tm2a(KOMP)Wtsi}/H
Alternative name	EPD0657_2_G02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ppt2^{tm2a(KOMP)Wtsi}
Gene/Transgene symbol	Ppt2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from KOMP ES clone EPD0657_2_G02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

increased blood urea nitrogen level / IMPC

MGI phenotypes (gene matching)

extramedullary hematopoiesis / MGI
abnormal urinary bladder urothelium morphology / MGI
abnormal liver morphology / MGI
enlarged liver / MGI
abnormal spleen morphology / MGI
enlarged spleen / MGI
muscle spasm / MGI
abnormal cerebral cortex morphology / MGI
abnormal cerebellar granule layer morphology / MGI
ataxia / MGI
limb grasping / MGI
abnormal pancreas morphology / MGI
premature death / MGI
abnormal brain morphology / MGI
decreased brain weight / MGI
abnormal bone marrow cell morphology/development / MGI
increased neuron apoptosis / MGI
enlarged pancreas / MGI
abnormal gastric gland morphology / MGI
increased spleen weight / MGI
abnormal retinal pigment epithelium morphology / MGI
abnormal exocrine pancreas morphology / MGI
hematopoietic system phenotype / MGI
jerky movement / MGI
increased macrophage cell number / MGI
abnormal spleen B cell follicle morphology / MGI
lipofuscinosis / MGI
abnormal pancreatic acinar cell zymogen granule morphology / MGI
abnormal cerebellum white matter morphology / MGI
abnormal pyloric gastric gland morphology / MGI
abnormal testis interstitial tissue morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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