A.B6-Prf1^tm1Sdz/Biat

Status	Available to order
EMMA ID	EM:07765
International strain name	A.B6-Prf1^tm1Sdz/Biat
Alternative name	AJ.PKO-/- or AJ.PKO-/+
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Prf1^tm1Sdz
Gene/Transgene symbol	Prf1

Information from provider

Provider	Steffen Frese
Provider affiliation	University of Bern
Genetic information	A/J mice which are susceptible for chemically (tobacco carcinogen NNK)-induced lung cancer were backcrossed for more than 10 generations with C57BL/6 perforin knock-out mice provided by Prof. Hengartner from University of Zurich.
Phenotypic information	Our data showed that these mice are more susceptible for chemically induced lung cancer (Frese-Schaper M. et al., Cancer Immunol Immunother, 12014).
Breeding history	The C57BL/6 mouse strain deficient in perforin was received from Hengartner's laboratory in 2009 and backcrossed for more than 10 generations to A/J background. A/J mice can be used as a model for chemically induced lung tumors.
References	Decreased tumor surveillance in perforin-deficient mice.;van den Broek M E, Kägi D, Ossendorp F, Toes R, Vamvakas S, Lutz W K, Melief C J, Zinkernagel R M, Hengartner H, ;1996;The Journal of experimental medicine;184;1781-90; 8920866 Influence of natural killer cells and perforin‑mediated cytolysis on the development of chemically induced lung cancer in A/J mice.;Frese-Schaper Manuela, Keil Andreas, Yagita Hideo, Steiner Selina Katja, Falk Werner, Schmid Ralph Alexander, Frese Steffen, ;2014;Cancer immunology, immunotherapy : CII;63;571-80; 24658838
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria
Animals used for archiving	homozygous A/J

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

Fatal post-viral neurodegenerative disorder / Orphanet_391343
Familial hemophagocytic lymphohistiocytosis / Orphanet_540

IMPC phenotypes (gene matching)

decreased mean corpuscular hemoglobin concentration / IMPC

MGI phenotypes (allele matching)

insulitis / MGI
decreased susceptibility to autoimmune diabetes / MGI
periinsulitis / MGI
increased incidence of induced tumors / MGI
impaired natural killer cell mediated cytotoxicity / MGI
decreased leukocyte cell number / MGI
decreased neutrophil cell number / MGI
decreased bone marrow cell number / MGI
ruffled hair / MGI
demyelination / MGI
weight loss / MGI
hypoactivity / MGI
hunched posture / MGI
increased circulating triglyceride level / MGI
abnormal body temperature homeostasis / MGI
abnormal T cell activation / MGI
brain inflammation / MGI
liver inflammation / MGI
abnormal lymph node morphology / MGI
abnormal spleen white pulp morphology / MGI
increased susceptibility to viral infection / MGI
decreased erythrocyte cell number / MGI
thrombocytopenia / MGI
lymph node inflammation / MGI
abnormal lymphocyte physiology / MGI
abnormal behavior / MGI
abnormal cytotoxic T cell physiology / MGI
decreased cytotoxic T cell cytolysis / MGI
pancytopenia / MGI
lethargy / MGI
increased circulating aspartate transaminase level / MGI
behavior/neurological phenotype / MGI
immune system phenotype / MGI
decreased body temperature / MGI
CNS inflammation / MGI
increased spleen red pulp amount / MGI
increased circulating tumor necrosis factor level / MGI
decreased interferon-gamma secretion / MGI
increased circulating interferon-alpha level / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-10 level / MGI
increased circulating interleukin-6 level / MGI
increased circulating interleukin-18 level / MGI
increased circulating interleukin-1 beta level / MGI
decreased NK cell degranulation / MGI
abnormal brain meninges morphology / MGI
increased susceptibility to viral infection induced morbidity/mortality / MGI
abnormal circulating cytokine level / MGI
abnormal circulating fibrinogen level / MGI
abnormal macrophage activation involved in immune response / MGI
increased circulating lactate dehydrogenase level / MGI
abnormal inflammatory response / MGI
increased B cell derived lymphoma incidence / MGI
premature death / MGI
increased susceptibility to infection / MGI
increased susceptibility to bacterial infection / MGI
abnormal leukocyte physiology / MGI
abnormal female reproductive system physiology / MGI
increased susceptibility to parasitic infection / MGI
increased interleukin-10 secretion / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased tumor latency / MGI
abnormal NK cell physiology / MGI
increased lymphoma incidence / MGI
abnormal circulating glucose level / MGI
decreased pancreatic beta cell number / MGI
increased physiological sensitivity to xenobiotic / MGI
increased T cell derived lymphoma incidence / MGI
increased lung adenocarcinoma incidence / MGI
increased sarcoma incidence / MGI
abnormal tumor morphology / MGI
increased tumor latency / MGI

MGI phenotypes (gene matching)

abnormal circulating glucose level / MGI
decreased leukocyte cell number / MGI
decreased neutrophil cell number / MGI
decreased bone marrow cell number / MGI
ruffled hair / MGI
demyelination / MGI
weight loss / MGI
hypoactivity / MGI
hunched posture / MGI
increased circulating triglyceride level / MGI
abnormal body temperature homeostasis / MGI
abnormal T cell activation / MGI
increased activated T cell number / MGI
abnormal inflammatory response / MGI
brain inflammation / MGI
liver inflammation / MGI
increased incidence of induced tumors / MGI
increased B cell derived lymphoma incidence / MGI
increased T cell derived lymphoma incidence / MGI
increased lung adenocarcinoma incidence / MGI
increased sarcoma incidence / MGI
premature death / MGI
no abnormal phenotype detected / MGI
abnormal lymph node morphology / MGI
abnormal spleen white pulp morphology / MGI
increased susceptibility to infection / MGI
decreased susceptibility to bacterial infection / MGI
increased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal leukocyte physiology / MGI
decreased erythrocyte cell number / MGI
decreased circulating alanine transaminase level / MGI
abnormal cytokine secretion / MGI
thrombocytopenia / MGI
decreased pancreatic beta cell number / MGI
abnormal tumor morphology / MGI
abnormal female reproductive system physiology / MGI
increased tumor growth/size / MGI
lymph node inflammation / MGI
abnormal lymphocyte physiology / MGI
insulitis / MGI
decreased susceptibility to autoimmune diabetes / MGI
abnormal behavior / MGI
increased susceptibility to parasitic infection / MGI
impaired natural killer cell mediated cytotoxicity / MGI
abnormal cytotoxic T cell physiology / MGI
decreased cytotoxic T cell cytolysis / MGI
pancytopenia / MGI
lethargy / MGI
increased circulating aspartate transaminase level / MGI
behavior/neurological phenotype / MGI
immune system phenotype / MGI
decreased body temperature / MGI
periinsulitis / MGI
CNS inflammation / MGI
decreased T cell apoptosis / MGI
increased spleen red pulp amount / MGI
increased circulating tumor necrosis factor level / MGI
increased tumor necrosis factor secretion / MGI
increased interferon-gamma secretion / MGI
decreased interferon-gamma secretion / MGI
increased circulating interferon-alpha level / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-10 level / MGI
increased circulating interleukin-6 level / MGI
increased circulating interleukin-18 level / MGI
increased circulating interleukin-1 beta level / MGI
increased interleukin-10 secretion / MGI
decreased NK cell degranulation / MGI
increased physiological sensitivity to xenobiotic / MGI
abnormal brain meninges morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased susceptibility to viral infection induced morbidity/mortality / MGI
increased tumor latency / MGI
abnormal circulating cytokine level / MGI
abnormal circulating fibrinogen level / MGI
decreased tumor latency / MGI
abnormal NK cell physiology / MGI
abnormal macrophage activation involved in immune response / MGI
increased circulating lactate dehydrogenase level / MGI
increased lymphoma incidence / MGI

Literature references

Decreased tumor surveillance in perforin-deficient mice.;van den Broek M E, Kägi D, Ossendorp F, Toes R, Vamvakas S, Lutz W K, Melief C J, Zinkernagel R M, Hengartner H, ;1996;The Journal of experimental medicine;184;1781-90; 8920866
Influence of natural killer cells and perforin‑mediated cytolysis on the development of chemically induced lung cancer in A/J mice.;Frese-Schaper Manuela, Keil Andreas, Yagita Hideo, Steiner Selina Katja, Falk Werner, Schmid Ralph Alexander, Frese Steffen, ;2014;Cancer immunology, immunotherapy : CII;63;571-80; 24658838

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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A.B6-Prf1tm1Sdz/Biat