C57BL/6NTac-B9d1^{tm1a(EUCOMM)Wtsi}/IcsOrl

Status	Available to order
EMMA ID	EM:07720
International strain name	C57BL/6NTac-B9d1^{tm1a(EUCOMM)Wtsi}/IcsOrl
Alternative name	EPD0060_2_H09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	B9d1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	B9d1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0060_2_H09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac, wild-type C57BL/6NTac
Stage of embryos	8-cell

Disease and phenotype information

MGI allele-associated human disease models

Meckel syndrome / DOID:0050778

Orphanet associated rare diseases, based on orthologous gene matching

Joubert syndrome / Orphanet_475
Meckel syndrome / Orphanet_564

IMPC phenotypes (allele matching)

abnormal startle reflex / IMPC
abnormal response to tactile stimuli / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal startle reflex / IMPC
abnormal response to tactile stimuli / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

lethality throughout fetal growth and development, complete penetrance / MGI
cleft palate / MGI
abnormal liver morphology / MGI
dextrocardia / MGI
telencephalon hypoplasia / MGI
exencephaly / MGI
microphthalmia / MGI
abnormal kidney morphology / MGI
thin myocardium / MGI
abnormal bile duct development / MGI
enlarged kidney / MGI
abnormal facial morphology / MGI
bile duct hyperplasia / MGI
holoprosencephaly / MGI
polycystic kidney / MGI
preaxial polydactyly / MGI
ventricular septal defect / MGI
abnormal renal tubule epithelial cell primary cilium morphology / MGI
perinatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
dilated nephron / MGI
renal tubule hypertrophy / MGI
decreased embryonic cilium length / MGI
decreased embryonic cilium number / MGI

MGI phenotypes (gene matching)

cleft palate / MGI
abnormal craniofacial morphology / MGI
polydactyly / MGI
abnormal liver morphology / MGI
dextrocardia / MGI
telencephalon hypoplasia / MGI
exencephaly / MGI
microphthalmia / MGI
abnormal blood vessel morphology / MGI
abnormal embryo development / MGI
abnormal limb morphology / MGI
abnormal cardiovascular system morphology / MGI
abnormal kidney morphology / MGI
abnormal hepatobiliary system morphology / MGI
thin myocardium / MGI
abnormal bile duct development / MGI
no phenotypic analysis / MGI
enlarged kidney / MGI
abnormal facial morphology / MGI
abnormal nervous system development / MGI
abnormal embryonic neuroepithelium morphology / MGI
bile duct hyperplasia / MGI
holoprosencephaly / MGI
polycystic kidney / MGI
preaxial polydactyly / MGI
ventricular septal defect / MGI
abnormal renal tubule epithelial cell primary cilium morphology / MGI
perinatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
dilated nephron / MGI
renal tubule hypertrophy / MGI
decreased embryonic cilium length / MGI
decreased embryonic cilium number / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6NTac-B9d1tm1a(EUCOMM)Wtsi/IcsOrl