C57BL/6NTac-Ttll1^{tm1a(EUCOMM)Wtsi}/IcsOrl

Status	Available to order
EMMA ID	EM:07712
International strain name	C57BL/6NTac-Ttll1^{tm1a(EUCOMM)Wtsi}/IcsOrl
Alternative name	EPD0066_2_A08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ttll1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Ttll1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0066_2_A08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac, wild-type C57BL/6NTac
Stage of embryos	8-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal tail movements / IMPC
increased startle reflex / IMPC
increased IgE level / IMPC
decreased hemoglobin content / IMPC
increased lean body mass / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
increased mean corpuscular hemoglobin concentration / IMPC
increased startle reflex / IMPC
increased lean body mass / IMPC
abnormal tail movements / IMPC
increased IgE level / IMPC
decreased hemoglobin content / IMPC

MGI phenotypes (gene matching)

abnormal spermatogenesis / MGI
decreased body weight / MGI
increased susceptibility to otitis media / MGI
rhinitis / MGI
sinus inflammation / MGI
male infertility / MGI
abnormal mucociliary clearance / MGI
abnormal respiratory sounds / MGI
abnormal tracheal ciliated epithelium morphology / MGI
asthenozoospermia / MGI
oligozoospermia / MGI
nervous system phenotype / MGI
decreased lean body mass / MGI
growth/size/body region phenotype / MGI
respiratory system phenotype / MGI
reproductive system phenotype / MGI
teratozoospermia / MGI
absent sperm flagellum / MGI
detached sperm flagellum / MGI
absent sperm head / MGI
short sperm flagellum / MGI
abnormal sperm midpiece morphology / MGI
abnormal respiratory motile cilium morphology / MGI
abnormal respiratory motile cilium physiology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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