C57BL/6NTac-Eif2b5^{tm1a(EUCOMM)Wtsi}/IcsOrl

Status	Available to order
EMMA ID	EM:07702
International strain name	C57BL/6NTac-Eif2b5^{tm1a(EUCOMM)Wtsi}/IcsOrl
Alternative name	EPD0116_1_C09
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Eif2b5^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Eif2b5
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0116_1_C09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Congenital or early infantile CACH syndrome / Orphanet_157713
Late infantile CACH syndrome / Orphanet_157716
Ovarioleukodystrophy / Orphanet_99853
Juvenile or adult CACH syndrome / Orphanet_157719
Cree leukoencephalopathy / Orphanet_99854

IMPC phenotypes (allele matching)

decreased granulocyte number / IMPC
decreased mean corpuscular volume / IMPC
decreased heart weight / IMPC
abnormal bone mineralization / IMPC
abnormal bone structure / IMPC
increased circulating creatinine level / IMPC
decreased circulating glucose level / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
increased bone mineral content / IMPC

IMPC phenotypes (gene matching)

decreased mean corpuscular volume / IMPC
increased bone mineral content / IMPC
abnormal bone mineralization / IMPC
decreased granulocyte number / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
abnormal bone structure / IMPC
decreased circulating glucose level / IMPC
increased circulating creatinine level / IMPC
decreased heart weight / IMPC

MGI phenotypes (gene matching)

abnormal forebrain morphology / MGI
abnormal myelination / MGI
demyelination / MGI
sporadic seizures / MGI
decreased body weight / MGI
abnormal retina morphology / MGI
ataxia / MGI
impaired coordination / MGI
premature death / MGI
abnormal astrocyte morphology / MGI
ectopic Bergmann glia cells / MGI
abnormal locomotor activation / MGI
abnormal microglial cell physiology / MGI
abnormal retinal outer nuclear layer morphology / MGI
abnormal retinal inner nuclear layer morphology / MGI
abnormal axon morphology / MGI
decreased percent body fat/body weight / MGI
brain vacuoles / MGI
abnormal brain white matter morphology / MGI
abnormal brain internal capsule morphology / MGI
thin retinal inner plexiform layer / MGI
abnormal physiological response to xenobiotic / MGI
abnormal astrocyte physiology / MGI
decreased oligodendrocyte number / MGI
abnormal cerebellum white matter morphology / MGI
decreased grip strength / MGI
increased oligodendrocyte number / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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