C57BL/6NTac-Ube2b^{tm1a(EUCOMM)Wtsi}/IcsOrl

Status	Available to order
EMMA ID	EM:07693
International strain name	C57BL/6NTac-Ube2b^{tm1a(EUCOMM)Wtsi}/IcsOrl
Alternative name	EPD0038_4_D01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ube2b^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Ube2b
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0038_4_D01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating phosphate level / IMPC
increased circulating alkaline phosphatase level / IMPC
abnormal behavior / IMPC
decreased grip strength / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased circulating phosphate level / IMPC
decreased basophil cell number / IMPC
increased leukocyte cell number / IMPC
increased lymphocyte cell number / IMPC
limb grasping / IMPC
enlarged heart / IMPC
abnormal gait / IMPC
preweaning lethality, complete penetrance / IMPC
increased circulating alkaline phosphatase level / IMPC
abnormal coat appearance / IMPC
decreased grip strength / IMPC
abnormal behavior / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased neutrophil cell number / IMPC

MGI phenotypes (gene matching)

abnormal spermatogenesis / MGI
male infertility / MGI
asthenozoospermia / MGI
oligozoospermia / MGI
decreased testis weight / MGI
abnormal male meiosis / MGI
reproductive system phenotype / MGI
teratozoospermia / MGI
abnormal spermatocyte morphology / MGI
abnormal male germ cell apoptosis / MGI
abnormal chiasmata formation / MGI
abnormal sperm head morphology / MGI
abnormal sperm midpiece morphology / MGI
abnormal double-strand DNA break repair / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

Search

INFRAFRONTIER^® and European Mouse Mutant Archive - EMMA^® are registered trademarks at the European Union Intellectual Property Office (EUIPO).