C57BL/6NTac-Lhx1tm1e(EUCOMM)Wtsi/IcsOrl

Status

Available to order

EMMA IDEM:07692
International strain nameC57BL/6NTac-Lhx1tm1e(EUCOMM)Wtsi/IcsOrl
Alternative nameEPD0243_4_E03
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolLhx1tm1e(EUCOMM)Wtsi
Gene/Transgene symbolLhx1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0243_4_E03. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NTac, wild-type C57BL/6N Tac
Stage of embryos8-cell

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased startle reflex / IMPC
  • decreased circulating alkaline phosphatase level / IMPC
  • increased CD8-positive, alpha-beta T cell number / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating alkaline phosphatase level / IMPC
  • increased startle reflex / IMPC
  • increased CD8-positive, alpha-beta T cell number / IMPC
MGI phenotypes (gene matching)
  • absent kidney / MGI
  • abnormal brain development / MGI
  • abnormal spinal cord morphology / MGI
  • abnormal female reproductive system morphology / MGI
  • abnormal male reproductive system morphology / MGI
  • decreased embryo size / MGI
  • incomplete embryo turning / MGI
  • abnormal allantois morphology / MGI
  • abnormal neural tube morphology / MGI
  • no abnormal phenotype detected / MGI
  • abnormal gastrulation movements / MGI
  • abnormal primitive streak formation / MGI
  • no phenotypic analysis / MGI
  • abnormal egg cylinder morphology / MGI
  • absent ovary / MGI
  • absent ureter / MGI
  • abnormal primitive node morphology / MGI
  • dilated allantois / MGI
  • small allantois / MGI
  • abnormal rostral-caudal axis patterning / MGI
  • agonadal / MGI
  • abnormal hindbrain development / MGI
  • absent testes / MGI
  • acephaly / MGI
  • perinatal lethality, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • absent metanephros / MGI
  • absent midbrain / MGI
  • embryonic-extraembryonic boundary constriction / MGI
  • absent forebrain / MGI
  • rostral-caudal axis duplication / MGI
  • absent prechordal mesoderm / MGI
  • abnormal primitive streak elongation / MGI
  • abnormal anterior head development / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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