C57BL/6NTac-Rnase10tm1a(EUCOMM)Hmgu/IcsOrl

Status

Available to order

EMMA IDEM:07690
International strain nameC57BL/6NTac-Rnase10tm1a(EUCOMM)Hmgu/IcsOrl
Alternative nameHEPD0528_6_A02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolRnase10tm1a(EUCOMM)Hmgu
Gene/Transgene symbolRnase10
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0528_6_A02. For further details on the construction of this clone see the page at the IKMC portal. Removal of the targeting cassette using Flp recombinase is required to convert the targeted into a conditional allele - more information on conversion to the b,c and d allele forms. Click here for more information on EUCOMM final vectors
Phenotypic informationPotential EUMODIC data in the Europhenome database
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased bone mineral density / IMPC
  • decreased locomotor activity / IMPC
  • decreased circulating triglyceride level / IMPC
  • increased lean body mass / IMPC
  • decreased CD4-positive, alpha-beta T cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • increased total body fat amount / IMPC
IMPC phenotypes (gene matching)
  • decreased locomotor activity / IMPC
  • decreased circulating triglyceride level / IMPC
  • decreased CD4-positive, alpha-beta T cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • increased total body fat amount / IMPC
  • decreased bone mineral density / IMPC
  • increased lean body mass / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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